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Pancreatic oxidative stress is the result of high fat-fructose diet consumption from birth to adulthood in male Wistar rats: 4-phenylbutyric acid moderates this effect
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Mina Sadat Izadi   , Homeira Zardooz |
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Abstract: (241 Views) |
Introduction: The high amount of micronutrients in blood circulation consequent to high calorie intake, impose a heavy load on the mitochondria, induces oxidative stress. Therefore, we examined the high fat-fructose diet (HFFD) consumption effects from birth to adulthood on pancreatic oxidative stress parameters. In addition, the efficacy of 4-phenylbutyric acid (4-PBA) to reverse the probable oxidative stress induction was assessed.
Methods and Materials: The dams with their pups were randomly allocated into the normal diet (ND) and HFFD groups. At the weaning, the male offspring were divided into ND-None, ND-DMSO, ND-4-PBA, HFFD-None, HFFD-DMSO, and HFFD-4-PBA groups, and fed on their diets for another five weeks. The drug (50mg/kg) was injected for ten days. At the end, after 16h fasting the rats were decapitated and the pancreas tissue were removed. Catalase (CAT) activity and glutathione (GSH) level were assayed. The malondialdehyde (MDA) level was determined in pancreas tissue homogenates using the commercial colorimetric kit (ZellBio, Germany) and Bradford method.
Results: The pancreatic CAT activity was increased in HFFD-DMSO (P<0.01), HFFD-None, and HFFD-4-PBA (P<0.001) compared to the ND-None. While in ND-4-PBA (compared to ND-None and ND-DMSO, P<0.05 and P<0.001) and HFFD-DMSO (compared to HFFD-None, P<0.001) there was a significant decrease. The pancreatic GSH level was increased in ND-DMSO (P<0.001) and HFFD-4-PBA (P<0.05). The pancreatic GSH level was decreased in ND-4-PBA compared to the ND-DMSO (P<0.001). The pancreatic MDA level was increased in HFFD-DMSO (P<0.01). While, it was decreased in HFFD4-PBA compared to the HFFD-DMSO (P<0.01).
Conclusion: Taken together, HFFD consumption from birth to adulthood induces pancreatic oxidative stress. While administration of 4-PBA reduces pancreatic oxidative stress markers.
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| Keywords: High fat-fructose diet, oxidative stress, 4-phenylbutyricacid |
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Full-Text [PDF 277 kb]
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Type of Study: Research |
Subject:
General
Received: 2024/01/29 | Accepted: 2023/08/1 | Published: 2023/08/1
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