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Long-term inorganic nitrate administration protects against myocardial ischemia-reperfusion injury in female rats
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Younes Yassaghi   , Sajad Jeddi , Nasibeh Yousefzadeh , Khosrow Kashfi , Asghar Ghasemi |
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Abstract: (293 Views) |
Introduction: The favorable effects of nitrate against myocardial ischemia-reperfusion injury (MIRI) have primarily been investigated in male rats and in short term. Here we determine the impact of long-term nitrate intervention on baseline cardiac function and the resistance to MIRI in female rats.
Methods and Materials: Female Wistar rats were randomly divided into 2 groups (n=14/group), untreated and nitrate-treated, that the latter consumed 100 mg/L sodium nitrate in drinking water for 9 months. At intervention end, levels of serum progesterone and nitric oxide metabolites (NOx), heart NOx concentration, and expressions of NO synthase isoforms (NOS), i.e., endothelial (eNOS), neuronal (nNOS), and inducible (iNOS), were measured. Isolated hearts were also exposed to ischemia, and cardiac function indices (CFI) were recorded. When the IR period ended, infarct size, NO metabolites, eNOS, nNOS, and iNOS expression were also measured.
Results: Nitrate-treated rats had higher serum progesterone (29.8%, P=0/013), NOX (31.6%, P=0/035), and higher heart NOX (60.2%, P=0/007), nitrite (131%, P=0/017), and eNOS expression (200%, P=0/005). Nitrate had no significant effects on baseline CFI but it increased recovery of LVDP (19%, P=0/020), +dp/dt (16%, P=0/006), –dp/dt (14%, P=0/014) and decreased LVEDP (17%, P<0/001) and infarct size (34%, P<0/001) after IR. After the IR, the two groups had significantly different heart nitrite, nitrate, NOx, and eNOS and iNOS mRNA expressions.
Conclusion: Long-term nitrate intervention increased the resistance to MIRI in female rats; this was associated with increased heart eNOS expression and circulating progesterone before ischemia and blunting ischemia-induced increased iNOS and decreased eNOS after MIRI.
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| Keywords: Nitrate, Nitric oxide, Female rats, Myocardial ischemia-reperfusion injury, Inducible nitric oxide synthase, Endothelial nitric oxide synthase |
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Full-Text [PDF 333 kb]
(36 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2023/12/27 | Accepted: 2023/08/23 | Published: 2023/08/23
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