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Investigating the protective effects of Dorema aucheri hydroalcoholic extract on antioxidant capacity and oxidative damage of pancreas in streptozotocin-induced diabetic rats
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Fariba Namdar   , Samira Yazdanimehr , Elham Rezazadeh , Mohammad Taghi Mohammadi |
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Abstract: (205 Views) |
Introduction: The hydroalcoholic extract of Dorema aucheri (DA) Bilhar (Umbelliferae) leaves, as a medicinal plant, has powerful antioxidant properties. Accordingly, the present study aimed to examine the protective effects of DA hydroalcoholic extract against oxidative damage of diabetic pancreas by potentiation of the antioxidant capacity in streptozotocin-induced diabetic rats.
Methods and Materials: Experiment was performed in four groups of male Wistar rats (each group; n=6), normal, diabetic and two treatment groups (normal and diabetic rats treated with DA). Rats were made diabetic by a single intravenous injection of streptozotocin (45 mg/kg) at the beginning of study. Treatment groups received orally DA hydroalcoholic extract at dose of 200 mg/kg/day. After eight weeks, the pancreas tissues were removed under deep anesthesia. After tissue homogenization, the contents of glutathione (GSH), NOx, and malondialdehyde (MDA) as well as superoxide dismutase (SOD) activity were assessed by biochemical methods.
Results: Blood glucose of diabetic rats was above 350 mg/dL. The MDA and NOx content of the homogenized pancreas significantly increased in diabetic rats by 92% and 28%, respectively. Diabetes also decreased the content of GSH content (32%) and SOD (68%) activity of pancreas. Treatment with DA hydroalcoholic extract noticeably decreased the MDA and NOx levels of diabetic pancreas. Moreover, DA hydroalcoholic extract significantly increased the GSH content and SOD activity of pancreas in treated diabetic rats.
Conclusion: Our findings revealed that DA hydroalcoholic extract was able to improve the uncontrolled hyperglycemia-induced oxidative damage of pancreas through potentiation of the antioxidant defense system in accompany with partial improvement of blood glucose.
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| Keywords: Diabetes mellitus, Dorema aucheri, Hyperglycemia, Oxidative damage, Antioxidant |
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Full-Text [PDF 260 kb]
(26 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/03/2 | Accepted: 2023/08/10 | Published: 2023/08/10
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