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Exosome therapy improved ovarian function in the rat model of premature ovarian insufficiency
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Nahideh Nazdikbin Yamchi   , Shahin Ahmadian , Halimeh Mobarak , Farhad Amjadi , Rahim Beheshti , Amin Tamadon , Reza Rahbarghazi , Mahdi Mahdipour |
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Abstract: (248 Views) |
| Introduction: Premature ovarian insufficiency (POI), is a reproductive system complication and characterized by alteration in sex hormones and ovarian tissue mis-function under the age of forty. Beside genetic background, several other reasons such as physiological and environmental factors were noted to promote POI conditions. New generation of therapeutics using cell and cell free products have brought regenerative potentials for vast ranges of health complexities including infertility. Exosomes are nono-sized extracellular vesicles secreted by different type of cells, contain numerus biological molecules that can harbor diverse signaling cascades in a paracrine manner to the recipient cells. In this investigation, rat model of POI was produced and confirmed using histopathological analysis. Subsequently, amniotic-fluid derived exosomes (AF-Exos) were enriched and transplanted directly into the ovarian tissue. Four weeks post treatment, rats were subjected to histopathological evaluation and mating trial. Microscopic findings illustrated that in POI rats, total number of atretic follicles were increased and in contrast the number of morphologically healthy follicles were decreased. After AF-Exos transplantation, however, general improvement in the number of follicles at different developmental stages were registered compared to the control POI rats coincided with the decrease pattern in the number of atretic follicles. Mating trial revealed higher numbers of births in the AF-Exos transplanted rats compared to the control group. Administration of AF-Exos seems to have rejuvenating properties in ovarian tissue of POI rats. Further investigations should elucidate the underlying mechanisms and safety of exosome therapy in clinical settings. |
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| Keywords: Premature ovarian insufficiency, exosome, ovarian, fertility preservation |
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Full-Text [PDF 279 kb]
(31 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/02/23 | Accepted: 2023/08/10 | Published: 2023/08/10
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