|
Protective effect of everolimus against 6-hydroxy dopamine-induced toxicity in SH-SY5Y cells
|
Hamid Reza Sadeghnia   , Saba Moini , Mohaddeseh Sadat Alavi , Roghayeh Rashidi |
|
|
|
Abstract: (253 Views) |
Introduction: Parkinson's disease is a degenerative neurological disorder of the nervous system that is associated with the loss of dopaminergic neurons. Everolimus, a selective inhibitor of mechanistic target of rapamycin (m-TOR) has been shown in studies to have neuroprotective effects. In this study, we aimed to investigate the protective effects of everolimus on the in vitro model of Parkinson's disease induced by 6-hydroxypopamine (6-OHDA) in SH-SY5Y dopaminergic cells.
Methods and Materials: SH-SY5Y cells were treated simultaneously with everolimus at concentrations of 1 to 100 nM and 6-OHDA (with 300 μM) for 24 h. Then, the cell survival, reactive oxygen species (ROS) and apoptosis were assessed using MTT, dichlorofluorescin diacetate (DCFDA) and propidium iodide (PI) assays.
Results: Data showed that simultaneous treatment of 6-OHDA-injured cells with everolimus significantly increased the cell survival. Everolimus also at concentrations of 10 and 25 nM significantly reduced ROS generation and apoptosis.
Conclusion: Everolimus decreased the toxicity of 6-OHDA in SH-SY5Y dopaminergic cells by reducing free radicals and inhibiting apoptosis. |
|
| Keywords: Everolimus, 6-Hydroxy dopamine, mechanistic target of rapamycin (m-TOR), Apoptosis, Oxygen free radicals, SH-SY5Y cells |
|
|
Full-Text [PDF 323 kb]
(31 Downloads)
|
Type of Study: Research |
Subject:
General
Received: 2024/02/13 | Accepted: 2023/08/23 | Published: 2023/08/23
|
|
|
|
|
|
|
| Send email to the article author |
|
|
|
| Add your comments about this article |
|
|
|
