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Evaluation of cardiac mitochondrial function in response of isolated cardiac muscle to ischemia-reperfusion injury in rats subjected to polycystic ovary syndrome
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Bita Zandi   , Kamran Rakhshan , Alireza Imani , Behjat Seifi |
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Abstract: (240 Views) |
Introduction: Today, polycystic ovary syndrome (PCOS) is considered as one of the risk factors for heart disease. Although high levels of androgens in PCOS cause dysfunction of mitochondria, the effect of PCOS on the cardiac cell’s mitochondria is unclear. In this study, we investigated the possible role of cardiac mitochondria in the response of isolated heart muscle to ischemia-reperfusion injury in the polycystic ovary syndrome model.
Methods and Materials: In this study 32 Wistar rats were divided into four groups of 8: control group (CTR), polycystic ovary group (PCOS), cardiac ischemia-reperfusion (IR) group, and polycystic ovary+cardiac ischemia-reperfusion (PCOS+IR) group. In order to induce the PCOS model, letrozole (1mg/kg/day) was administered orally to the animals for 4 weeks. Then the heart of the animals were removed from the body and transferred to the Langendorff apparatus through cannulation in the aorta. Ischemia was induced for 30 minutes by ligation of the left ventricular anterior descending artery (LAD). Opening this artery provides to restore blood flow (reperfusion) for 60 minutes. At the end of the study, the size of the infarction (TTC staining), histopathological changes (H&E staining), and the amount of mitochondrial fission factor (DRP-1) were evaluated at cardiac tissue.
Results: The size of the infarction and the amount of DRP-1 protein in the ischemia-reperfusion (IR) group has a significant increase compared to CTR group (P<0.05). These variables also significantly increase in the PCOS+IR group compared to the IR group (P<0.05). Histopathological examination showed destructive tissue changes in the ischemia-reperfusion groups (PCOS+IR, IR) compared to the control group, and the degree of these changes in the PCOS+IR group is more severe than the IR group (P<0.05).
Conclusions: It seems that the induction of polycystic ovary syndrome (PCOS), probably through mitochondrial damage in cardiac cells leads to a greater susceptibility of the heart to ischemia-reperfusion injury.
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| Keywords: Polycystic ovary syndrome, ischemia-reperfusion injury, mitochondrial fission factor |
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Full-Text [PDF 322 kb]
(26 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/02/11 | Accepted: 2023/08/23 | Published: 2023/08/23
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