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Anticancer effect of epigallocatechin gallate loaded nanoparticles on head and neck cancer cell line
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Zahra Khatibzadeh   , Elham Sadat Afraz , Sohrab Kazemi , Marjan Bahraminasab , Mohadeseh Arabhalvaee , Samaneh Arab |
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Abstract: (243 Views) |
Introduction: The current approach to the treatment of head and neck cancer due to recurrence, resistance to drugs, and side effects has necessitated the need for more efficient treatment methods. Epigallocatechin gallate is the most active catechin in green tea, which has been studied for its anti-cancer effects. Nevertheless, it has a short half-life and stability with low bioavailability. The use of nanocarriers can overcome these deficiencies. The purpose of this study was to investigate the anti-cancer effect of nanoparticles containing epigallocatechin gallate on head and neck cancer cells (TSCC-1).
Methods and Materials: First, nanoparticles loaded with epigallocatechin gallate were made, and then the nanoparticles were characterized by dynamic light scattering (DLS), zeta potential, Fourier transform infrared spectroscopy (FTIR), and field emission electron microscopy (FE-SEM). Furthermore, the drug release and loading were measured. The toxicity of nanoparticles containing epigallocatechin gallate on TSCC-1 cancer cell line by MTT test, colony formation, the apoptosis rate, and the expression of BAX and BCL2 genes by PCR test were analyzed.
Results: The results of the FTIR test confirmed the presence of polyethylene glycol nanoparticles containing epigallocatechin gallate. The nanoparticles showed sustained release of the drug. Moreover, the MTT test showed significant cytotoxicity of the nanoparticles on TSCC-1 cells. No colony formation showed in the groups treated with nanoparticles containing epigallocatechin gallate compared to the control group. The results of apoptosis showed the induction of delayed apoptosis in the group treated with nanoparticles. Furthermore, the expression level of BCL2 gene decreased significantly and the level of BAX gene increased, however, it was not statistically significant for BAX gene.
Conclusion: The results suggest that polyethylene glycol nanoparticles loaded with epigallocatechin gallate have a significant anti-cancer effect (TSCC-1) and they have the potential to treat squamous cell carcinoma in the head and neck. |
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| Keywords: Squamous cell carcinoma of head and neck, Epigallocatechin gallate, nanoparticles, green tea |
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Full-Text [PDF 268 kb]
(35 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/02/10 | Accepted: 2023/08/23 | Published: 2023/08/23
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