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Overexpression of miR-149-5p Attenuates Middle Cerebral Artery Occlusion Injury by Targeting Faslg
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Samira Vahidi   , Mohammad Reza Bigdeli , Mehrdad Roghani , Hosein Shahsavarani |
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Abstract: (244 Views) |
Introduction: Stroke is a leading cause of death and disability worldwide. Many patients still do not achieve optimal outcomes despite the advances in treatment methods. MicroRNAs are potential candidates for pre-treatment or treatment of ischemic stroke complications, as they often regulate multiple genes involved in various pathogenic processes that lead to stroke. MiR-149 can influence several genes that affect either cell growth or death, but its therapeutic potential for stroke-related neurological impairments has not been fully explored. This study investigated the effects of miR-149-5p overexpression on middle cerebral artery occlusion (MCAO) injury in rats and explored the underlying mechanisms.
Methods and Materials: Rats were randomly divided into sham, LV-Control, LV-miR-149 group, and miR-149-5p mimic. MiR-149-5p mimic or LV-miR-149 was injected into the brain's lateral ventricle before MCAO. Neurological deficits, infarct volume, brain edema, and Faslg expression were evaluated 24 hours after MCAO.
Results: MiR-149-5p overexpression significantly reduced neurological deficits, infarct volume, and brain edema in MCAO rats compared with the control group. MiR-149-5p overexpression also downregulated Faslg expression in the ischemic penumbra.
Conclusion: MiR-149-5p overexpression attenuates MCAO injury by targeting Faslg and inhibiting apoptosis so miR-149-5p may be a potential therapeutic target for ischemic stroke. |
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| Keywords: miR-149-5p, Faslg, ischemic stroke, apoptosis |
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Full-Text [PDF 255 kb]
(35 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/02/9 | Accepted: 2023/08/23 | Published: 2023/08/23
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