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Synergistic effects of Nepeta menthoides and Melissa officinalis aqueous extracts on depressive-like behaviors in reserpinised-mice
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Batool Rahmati   , Sedighe Talebi , Seyed Abbas Hasheminejad , Masoumeh Jorjani , Fatemeh Emadi , Mohsen Naseri |
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Abstract: (263 Views) |
Introduction: Nepeta menthoides Boiss & Buhse and Melissa officinalis are extensively used in Persian medicine for the treatment of depression. Considering the active ingredients and main phenolic compounds of these plants and possible synergistic effects, this study examined antidepressant and antioxidant activities of aqueous extract of N. menthoides (NM) and M. officinalis (MO) in reserpinized mice alone and combination.
Methods and Materials: Mice were pretreated orally for one-week with normal saline (10 ml/kg), fluoxetine (20 mg/kg), imipramine (10 mg/kg), NM (50-400 mg/kg), MO (150-750 mg/kg) and combination (NM 50 mg/kg with MO 150 mg/kg). Antidepressant effects were evaluated using forced swim, tail suspension, and open field tests 24 hours after reserpine injection (4 mg/kg, i.p.) on 8th day. The amounts of active components in the extracts and catalase as a brain oxidative stress were measured with ELISA.
Results: Data showed that this combination produced a synergistic action and a significant increase in brain catalase activity (p<0.05). HPLC results showed that rosmarinic acid contents in MO and NM were 6.42±1.1 and 11.03±2.16 mg/g of dried extract, respectively. Total flavonoid and phenolic contents of MO were higher than NM.
Conclusion: The findings suggest that the present combination produces an antidepressant-like effect, which is possibly triggered by its anti-oxidant properties.
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| Keywords: Nepeta menthoides, Melissa officinalis, Pharmacological synergy, Antidepressant effects, Catalase activity, Persian medicine |
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Full-Text [PDF 272 kb]
(37 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/02/8 | Accepted: 2023/08/23 | Published: 2023/08/23
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