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Protective effects of calorie restriction and 17-beta estradiol against cardiac inflammation and AKI in ovariectomized obese rats
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Mohammad Khaksari   , Zahra Hajializadeh , Fatemeh Darvishzadeh |
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Abstract: (222 Views) |
Introduction: Lifestyle and eating habits (including obesity) affect the health and function organs, including the heart and kidneys. On the other hand, it has been found that estrogen has a protective role in acute kidney injury (AKI) and also a reduction in daily caloric consumption delays the onset of diseases. The protective effects of calorie restriction (CR) and 17-beta estradiol on cardiac inflammation and AKI indices were studied in female rats.
Methods and Materials: Female Wistar rats were divided into two separate groups, sham and ovariectomized, and received a standard diet, a 60% high-fat diet, or were subjected to caloric restriction (30%) for 16 weeks. Subsequently, ovariectomized animals were administered 17-beta-estradiol (E2) intraperitoneally every four days for four weeks. Real-time PCR method was performed in all heart groups and AKI indices were measured.
Results: HFD increased IL-6 and TNF-α gene expression, while decreased IL-10 expression in ovariectomized rats. In addition, HFD decreased the gene expression of estrogen receptor α (ERα) and estrogen receptor β (ERβ) in these animals. On the other hand, obese rats without ovaries showed a decrease in IL-6 and TNF-α levels, as well as an increase in IL-10 gene expression, ERα and ERβ gene expression in response to CR and E2. After AKI, CR caused a decrease in renal injury indices, a decrease in oxidative stress, TGF-β1 and an increase in SIRT1, which were reduced by ovariectomy.
Conclusion: The current study established that CR and E2 are capable of mitigating cardiac inflammation and reduction of AKI indicators in the postmenopausal condition by acting through the mediation of estrogen receptor Reduction of AKI indicators.
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| Keywords: Obesity, 17-beta estradiol, Calorie restriction, Cardiac inflammation, AKI |
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Full-Text [PDF 331 kb]
(29 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/02/4 | Accepted: 2023/08/23 | Published: 2023/08/23
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