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Neuroprotective effects of coenzyme Q10 on neurological scores, permeability of the blood-brain barrier and cerebral edema after stroke induction, acute concussion by Marmaro method in male rats: a behavioral, biochemical and histological study
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Ali Siahposht-Khachaki   , Anahita Mohsenpour , Kimia Ramezani , Paniz Salek |
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Abstract: (244 Views) |
Introduction: TBI is one of the main causes of deaths and chronic disability worldwide. Overall, TBI (traumatic brain injury) is a growing epidemy in 3rd-world nations and developing countries, which use road transportation and motor vehicles more. The intention of this research is studying the impacts of Neuroprotective Coenzyme Q10, which is a part of mitochondrial electron chain, an effective antioxidant, which plays a role in membrane consolidation. The studies have shown that Co Q10 is effective in treatment of ischemia injury and blood recirculation. The research has been done on neurological scores, health of blood-brain barrier, and brain edema as a result of brain trauma in male rats on a behavioral, biochemical and histological basis.
Methods and Materials: In this study, 56 of white male rats’ heads, each weighing 250-330 g, and 6 to 8 heads are used in each group. Before the operation, the animals get anesthesia by the use of Ketamine (50- mg/kg) and Xylazine (10 mg/kg). Also, they get Analgesia by the use of Cocktail Midazolam (intra-dermal, 2mg/kg) and Medetomidine (1mg/kg). Then, the heads are fixed in Stereotaxic in order to find out the yes/no outcome.A few minutes later, various doses of Co-enzyme Q10 are intraperitoneally injected.
Results: Neurological scores were measured before, at the time and first, second and third day after the injury. Analyze of statistics and two-way AVONA test show that Co-enzyme doses of 10, 20 and 40 mg/kg have caused the decrease of neurological scores. However, intra peritoneal prescription of doses of 10 and 20 mg/kg has caused an increase in neurological scores of the specific groups. The scores did not change in the dose of 40 mg/kg.Times before the injury, the time of consciousness after the injury and 24, 48 and 72 hours after the injury and also, Beam walk and Beam balance are administered. After 72 hours, Elisa test is done with the CSF. Then, 200 microliter of CSF Fluid from Cisterna Magna is taken and the color of CSF fluid can be observed.
Conclution: Prescription of 10 mg and 20 mg doses of Co-enzyme Q10 causes improvement in neurological statistics and balance factors. Also, prescription of 25 mg dose reduces stress, mental dysfunction and also, reduces the drivers of brain inflammation and restructures morphological changes in dead neurons. As a result, this medication can have protective impacts on neuronal cells and TBI which is caused by injury.
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| Keywords: Coenzyme Q10, Neurological scores, Traumatic brain injury, TBI, CSF |
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Full-Text [PDF 356 kb]
(36 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/02/2 | Accepted: 2023/08/23 | Published: 2023/08/23
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