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Effect of resveratrol on chronic cerebral hypoperfusion-induced neurological damages in rats
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Mohabbat Jamhiri   , Mansoureh Eslami , Fatemeh Safari , Fatemeh Zare Mehrjardi , Jalil Alizadeh Ghalenoei |
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Abstract: (230 Views) |
Introduction: Chronic cerebral hypoperfusion (CCH) is a common cause of cerebral small vascular disease which leads to memory loss. Myelin-associated Lingo-1/NgR1 signaling has crucial roles in modulation of synaptic plasticity, and thereby cognitive function. Resveratrol (RES), a natural compound with anti-oxidant properties found mainly in many grapes, exerts neuroprotective activity against cerebral ischemia. The purpose of this study was to investigate the effects of Resveratrol on neurological damages caused by chronic cerebral hypoperfusion in rats with focusing on evaluaton of Lingo-1/NgR1 expression.
Methods and Materials: Male Wistar rats were randomly divided into 5 experimental groups: Control (Ctl), Sham vehicle (Sham), Ischemia (ISC), ISC + RES and RES. Resveratrol was administered at 5 mg/kg intraperitoneally for five weeks .Chronic cerebral hypoperfusion model was established by 2-vessel occlusion (2VO). The learning and memory of the rats were measured using the Morris water maze test. The protein and mRNA levels of Lingo-1/NgR1 were detected by western blot and real time RT-PCR techniques, respectively.
Results: Compared with those results of ISC group, ISC+RES and RES groups markedly improved learning impairment in rats. Spatial memory results showed that the average time spent and the distance traveled in the target quadrant increased in the ISC+RES and RES groups compared to the ISC group. Resveratrol could significantly reduced Lingo1 and NgR1 protein expression in RES and ISC+RES groups compared to the ISC group (P˂0.05). Also, the mRNA level of Lingo-1 and NgR1 in the ISC+RES group showed a significant decrease compared to the ISC group (P˂0.05 and P˂0.01, respectively).
Conclusion: Based on our results, we propose that RES may improve cognitive impairment induced by chronic cerebral hypoperfusion through inhibiting the Lingo-1/NgR1 pathway to facilitate neural plasticity. Thus, RES can be used as a potential neuroprotective agent in chronic cerebral hypoperfusion therapy.
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| Keywords: Chronic cerebral hypoperfusion, Resveratrol, Lingo-1, NgR1 |
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Full-Text [PDF 352 kb]
(32 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/01/30 | Accepted: 2023/08/1 | Published: 2023/08/1
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