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Identification of key microRNAs and protein-protein interactions of the testis-expressed (TEX) proteins implicated in the non-obstructive azoospermia
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Zahra Hasani Mahforoozmahalleh   , Danial Hashemi Karoii |
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Abstract: (250 Views) |
Introduction: Non-obstructive azoospermia (NOA) is diagnosed with a lack of sperm in ejaculation and is definitely one of the main causes of male infertility. As it can be influenced by multiple genes, detecting chromosomal abnormalities and recognizing its genetic components are among the important goals of researchers to facilitate new and better ways of treatment. Human testis-expressed (TEX) genes are expressed predominantly in germ cells, thus they play significant roles in spermatogenesis and fertilization in humans. We want to recognize which cluster of TEX genes are involved in NOA.
Methods and Materials: In the present study, microRNA databases (miRBase, TarDB, and miRVIT) and protein-protein interaction (PPI) like STRING are used to predict and find hub genes by some microRNA, Enrichr database and Cytoscape software.
Results: We selected TEX11 and TEX14 from 110 Homo Sapiens-related genes uncovered in NCBI’s gene database. Genes are enriched and hsa-miR-563, hsa-miR-3622a-5p, hsa-miR-4796-5p, hsa-miR-645, hsa-miR-2964a-5p and hsa-miR-556-5p may be changed in NOA. Three hub genes were determined in the PPI network including MSH4, HFM1, and RNF212. These genes associated with TEX11 and TEX14 are involved in NOA. Gene ontology reveals that mitotic spindle assembly checkpoint signaling (GO: 0007094) and homologous chromosome pairing at Meiosis are involved in biological processes (GO: 0007129). On the other hand, molecular function showed that these genes are involved in Kinase Binding (GO: 0019900) and Protein Kinase Binding (GO: 0019901). Other miRNA analyses demonstrated that these predicted miRNAs can interact with TEX11 and TEX14 and change the gene ontology and signaling pathways.
Conclusion: By discovering the microRNA interaction, it may also be possible to determine the activity of the hub proteins network with TEX11 and TEX14 engaged in NOA. These miRNA-PPI targets could be precious biomarkers for NOA.
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| Keywords: non-obstructive azoospermia, TEX11, TEX14, miRNA, biomarkers |
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Full-Text [PDF 346 kb]
(32 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/01/29 | Accepted: 2023/08/1 | Published: 2023/08/1
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