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Antioxidant properties of Pistacia vera against the effects of phenylhydrazine-induced hemolytic anemia on male fertility in Mice
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Soudeh Khanamani Falahati-pour   , Arezoo Khoradmehr , Fatemeh Baghery , Fatemeh Amin , Najmeh Parvaz , Soheila Pourmasumi , Erfan Shahabinejad |
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Abstract: (288 Views) |
Introduction: Anemia can harm the reproductive system due to high free radical production, lipid peroxidation, and decreased glutathione levels, whereas pistachio pericarp is renowned for its antioxidant properties. A model of mice was used to assess the protective effects of aqueous extract of pistachio (Pistacia vera) versus oxidative stress (OS) caused by phenylhydrazine (PHZ)-induced hemolytic anemia on sperm parameters, sex hormone, and OS markers.
Methods and Materials: Forty-eight male mice were divided as (n=8): control, PHZ, PHZ+20Pis (aqueous extract of pistachio pericarp), PHZ+80Pis, PHZ+160Pis, and 80Pis group. A phenylhydrazine dose of 80 mg/kg/48 hours was given to the experiment groups, as well as a pistachio dose of 20, 80, and 160 mg/kg/day, intraperitoneally. After 35 days, all mice were sacrificed and sperm count, motility, morphology, and viability were evaluated as well as sperm chromatin quality. Hormonal (FSH, LH, testosterone) and antioxidant levels were analysed using standard kits.
Results: A significant reduction in the sperm parameters (normal morphology, count, and motility), chromatin quality (AB, TB, CMA3), and sex hormones (FSH, LH, Testosterone) obtained in the PHZ groups compared to control group. Also, there was a considerable decrease in spermatogonia cell number, spermatid, tubule count, seminiferous diameters, and seminiferous area in the PHZ group in comparison with the control group. The highest level of antioxidant contents was observed in the Pis group.
Conclusion: A high level of antioxidant components with therapeutic properties may explain the protective effect of P.vera extract versus PHZ induced anemia on the male reproductive system in mice.
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| Keywords: Anemia, Pistacia vera, Phenylhydrazine, Male mice, Antioxidant |
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Full-Text [PDF 265 kb]
(41 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/01/13 | Accepted: 2023/08/23 | Published: 2023/08/23
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