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Exploring A robust Animal Model for Osteoarthritis
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Soroush Tammari   , Maryam Vaseli Khabbaz , Payman Raise-Abdullahi , Ali Ghanbari , Ali Rashidipour , Davood Nasrabadi |
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Abstract: (294 Views) |
Introduction: Osteoarthritis (OA) is a severe disorder of the diarthrodial joints and is characterized by chronic degeneration. It is the most prevalent type of arthritis worldwide and can lead to disability due to the associated pain. There are a few pharmacological OA treatment choices but no unequivocal medicines for it are available. on the other hand, The need for a successful treatment reflects the required better understanding of the pathophysiology and etiopathology of OA. Animal models have played a key role in achieving this goal. According to the research, intra-articular injection of collagenase could be a significant indicator because it induces articular degeneration by digesting collagen from cartilage and causing articular instability.
Methods and Materials: In this regard, 5 male Wistar rats were selected. Collagenase (250 μl) and chondroitinase (150 μl) were injected inter-articularly into their right knees over the course of 3 shifts (250 µl per shift). As a control sample, their left knees were inspected.
Results: After eight weeks of the primary dose injection, a Paw-Print behavioral test is performed to assess and compare pain between the patient's paw and the control. Additionally, the results of serology and histology tests confirmed the creation of the OA model.
Conclusion: To the best of our knowledge, this is the first study to investigate intra-articular injection of collagenase and chondroitinase for creating in vivo models of OA simultaneously. Since the clinical changes observed were comparable to those described in patients with OA, this model can be useful for exploring the pathophysiology of OA and developing treatments for this disease.
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| Keywords: Osteoarthritis, Collagenase, Chondroitinase, Rat model, Paw-Print behavioral |
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Full-Text [PDF 317 kb]
(43 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/01/11 | Accepted: 2023/08/23 | Published: 2023/08/23
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