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Cardioprotective effects of hydroalcoholic extract of Pinus eldarica bark on adrenaline-induced myocardial infarction in rats
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Mahnaz Halvaee varnosfaderani   , Leila Safaeian , Mahdi Amindeldar , Behzad Zolfaghari , Zahra Haghighatian |
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Abstract: (269 Views) |
Introduction: Myocardial infarction is one of the most dangerous cardiovascular diseases. Plants belonging to the genus Pinus are rich in polyphenolic compounds and possess beneficial cardiovascular effects. In the present study, the effect of hydroalcoholic extract of the bark of Tehran pine tree (Pinus eldarica) on adrenaline-induced myocardial infarction in Wistar rats was investigated.
Methods and Materials: Total phenolic content of P. eldarica extract was determined using Folin-ciocalteu method. Pretreatment was done by oral administration of 100, 200 and 400 mg/kg of extract for 16 days. Myocardial infarction was induced by subcutaneous injection of adrenaline (2 mg/kg) on 15th and 16th days. At the end of the treatment period, laboratory tests as evaluation of ECG, assessment of serum parameters of heart damage including troponin I, aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and creatine phosphokinase (CK-MB) as well as malondialdehyde (MDA) and total antioxidant capacity (FRAP) were performed and the heart of the animals was evaluated histologically.
Results: Total phenolic content of hydroalcoholic extract of P. eldarica bark was determined as 560.65+44 mg/g of extract as gallic acid equivalent. Administration of adrenaline resulted in the significant changes in ECG as ST segment elevation, increase in R-R interval, and decrease in heart rate, as well as elevation in LDH, troponin I, AST and MDA, decrease in FRAP, a significant increase in heart weight, and pathological changes in the heart tissue. P. eldarica bark extract at the doses of 200 and 400 mg/kg significantly prevented the changes in ECG and reduced the serum markers of heart damage and MDA, increased FRAP level and improved the heart histopathologic changes.
Conclusion: This study suggests the beneficial cardioprotective and antioxidant effects of hydroalcoholic extract of P.eldarica bark in adrenaline-induced myocardial infarction.
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| Keywords: Pinus eldarica, Myocardial infarction, Antioxidant |
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Full-Text [PDF 343 kb]
(49 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/01/11 | Accepted: 2023/08/23 | Published: 2023/08/23
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