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Effect of long-term nitrate administration on sialin gene expression in male Wistar rats with type 2 diabetes
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Nasibeh Yousefzadeh   , Sajad Jeddi |
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Abstract: (285 Views) |
Introduction: Increased sialin expression in the main tissues of rats with type 2 diabetes (T2D) is accompanied by decreased nitrate and nitrite concentrations in these tissues. It is hypothesized that increased sialin expression may act as a compensatory mechanism to counteract reduced nitric oxide (NO) bioavailability in rats with T2D. This study aimed to assess the changes in sialin mRNA expression in the soleus muscle, stomach, adrenal glands, liver, and epididymal adipose tissue (eAT) of rats with T2D following nitrate administration at a dose of 100 mg/L for 6 months.
Methods and Materials: Rats were divided into two groups (n=10/group): T2D and T2D +nitrate. At month 6, samples from the main tissues including the soleus muscle, stomach, adrenal glands, liver, and eAT were used for measurements of the expression of the sialin using real-time PCR. NO metabolites (nitrate+nitrite= NOx) were measured by the Griess method at the end of the study.
Results: T2D+nitrate group had higher values of nitrate in the soleus muscle (222%, P=0/0075), stomach (67%, P=0/0252), adrenal glands (95%, P=0/0642), however, no changes were observed in the liver, and eAT. Compared to the diabetic rats, the T2D +nitrate group had higher values of nitrite levels in the soleus muscle (85%, P=0/0237), and adrenal glands (90%, P=0/0540). Nitrite values in the stomach and liver, and eAT were similar between diabetic and T2D +nitrate rats. Nitrate administration decreased the mRNA expression of sialin in the soleus muscle (0/21-fold, P<0/001), stomach (0/37-fold, P=0/002), and liver (0/21-fold, P<0/001).
Conclusion: These findings confirm our hypothesis about a compensatory mechanism of increased sialin expression to counteract reduced NO bioavailability in rats with T2D by decreasing sialin expression in main tissues of rats with T2D following nitrate administration.
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| Keywords: Nitrate, Nitrite, Type 2 diabetes, Sialin, Nitrate transporter |
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Full-Text [PDF 255 kb]
(35 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2023/12/29 | Accepted: 2023/08/23 | Published: 2023/08/23
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