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The evaluation of the effects of mesenchymal stem cell transplantation on the hormonal, mitochondrial and structural function of ovarian tissue in letrozole-induced polycystic ovary syndrome rat model
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Arash Abdi   , Behjat Seifi , Mina Ranjbaran , Fardin Amidi |
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Abstract: (290 Views) |
Introduction: Polycystic ovary syndrome (PCOS) is one of the most common endocrinology diseases in women's lives and is one of the main factors that account for infertility and irregular ovulation and increased levels of androgens. Stem cell therapy has recently been proposed as a method of treating a variety of disorders such as PCOS. Because Adipose Mesenchymal Stem Cells (AMSC) have high availability, easy access, good proliferation and lower immunological rejection, the objective of this study was to evaluate the effect of AMSC on the treatment of PCOS in rats.
Methods and Materials: In this experimental study, 18, 8-week-old female rats weighing approximately 180-200 g were randomly divided into three equal groups; the control group received 0.5% w/v carboxymethyl cellulose by gavage; the PCOS group received letrozole 1 mg/kg, daily in 0.5% carboxymethyl cellulose for 21 days by gavage. In the PCOS+AMSC group rats received 1 × 106 MSCs/rat (i.p., in day 28). At the end of the study, body weight, ovarian functional parameters, serum hormone levels and mitochondrial dynamics genes were assessed.
Results: Transplantation of AMSC reduced body weight, testosterone level, ovarian cystic, atretic follicle, and downregulated expression in Dynamin-related protein1, Mitofusins2 and increased estrogen level significantly compared to PCOS group (P<0/05).
Conclusion: AMSC Transplantation in PCOS rats with mitochondrial dynamic imbalances could be effective on ovarian structural changes and serum hormone levels. Thus, AMSC treatment mayconsider as a novel therapeutic strategy for PCOS.
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| Keywords: Mesenchymal Stem Cells, PCOS, mitochondrial dynamic |
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Full-Text [PDF 321 kb]
(45 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2023/12/29 | Accepted: 2023/08/23 | Published: 2023/08/23
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