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Effect of Black Radish Hydro Alcoholic Extract on Liver's Enzymes and Histology in Small Laboratory Mice
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Amir Hossein Mellat   , Amir Amani , Elham Moghtadaei khorasgani , Ashkan Sadeghi |
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Abstract: (283 Views) |
Introduction: The liver is the second largest organ (after the skin) and the largest gland in the body. Extensive functional storage of the liver obscures the clinical effect of early-stage liver injury. Injuries followed by hepatic necrosis lead to elevated serum levels of specific liver enzymes in the blood.Black radish is known by the scientific name Raphanus sativus L. var niger. The properties of black radish on the digestive system and especially to regulate bile function have been well known for many years.
Methods and Material: In order to test 50 adult female mice in 5 groups of ten; Control and three treatment groups were analyzed. Treatment groups with doses of 50, 100 and 150 mg/kg of hydroalcoholic extract of black horseradish were treated 10 times in between and intraperitoneally. After 20 days, liver enzyme levels ALT, AST and ALP were measured through blood tests. After relaxation, liver extraction and fixation in formalin. For histopathological examinations, liver tissue of mice was examined.
Results: The levels of triglycerides, ALT and ALP between the treated groups at a dose of 100 mg/kg and 150 mg/kg of black radish hydroalcoholic extract were higher than the control group and the treated group at a dose of 50 mg.
Conclusion: According to the enzymatic and pathological results. It can be concluded that consumption of black radish extract depends on high doses of toxic effects on liver tissue and based on the results of the above study, it can be said that the dose is 50 mg/ml of black radish extract can be introduced as a more appropriate dose than doses of 100 and 150 mg/kg.
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| Keywords: Raphanus Sativus, Liver, Liver enzymes |
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Full-Text [PDF 254 kb]
(40 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2023/12/17 | Accepted: 2023/08/23 | Published: 2023/08/23
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