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Investigating the effect of empagliflozin on biochemical factors of testicular tissue in rats with type 2 diabetes
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Mina Kiani   , Malek Soleimani Mehranjani , Seyed Mohammad Ali Shariatzadeh |
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Abstract: (272 Views) |
Introduction: Empagliflozin is a new drug that reduces blood sugar and insulin resistance by inhibiting the sodium-glucose cotransporter 2 enzyme and increasing the proliferation of beta cells. In type 2 diabetes (T2DM), insulin resistance increases the level of testicular inflammatory factors, and long-term hyperglycemia affects cholesterol levels and testicular testosterone synthesis. Considering the effect of empagliflozin on reducing insulin resistance, the aim of this study is to investigate the effect of empagliflozin on the level of pro-inflammatory factor Interlukin-6 (IL-6), Tumor necrosis factor (TNF-α), cholesterol and testicular testosterone in diabetic rats.
Methods and Materials: 18 adult male rats were divided into control, diabetic (streptozotocin (65 mg/kg) and nicotinamide (110 mg/kg), intraperitoneal injection) and diabetic+Empagliflozin group (10 mg/kg, gavage, 8 weeks). After dissecting the rats, the left testis was separated and stored at -80°C for further assessment of biochemical factors. Testicular levels of IL-6, TNF-α, and testosterone were measured by the ELISA method, and testicular cholesterol was measured by spectrophotometer.
Results: A significant increase in the mean level of IL-6 and TNF-α (P˂0.001) and a significant decrease in the mean level of cholesterol (P˂0. 05) and testosterone (P˂0.001) were observed in the diabetic group compared to the control group. In the diabetic + Empagliflozin group, a significant decrease in the level of IL-6 (P˂0.01) and TNF-α (P˂0.001) was observed compared to the diabetic group, while no significant difference was observed in the level of cholesterol and testosterone (p˃0.05).
Conclusion: Our results showed that Empagliflozin reduces testicular inflammation caused by diabetes but has no effect on testicular testosterone synthesis.
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| Keywords: Type 2 diabetes, Empagliflozin, IL-6, TNF-α, Testosterone |
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Full-Text [PDF 317 kb]
(48 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2023/12/17 | Accepted: 2023/08/23 | Published: 2023/08/23
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