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Age-dependent impacts of Tamoxifen on brain leptin responsiveness in young female high-fat diet-feeding mice
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Zeinab Farhadi   , Mohammad Khaksari , Hossein Azizian |
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Abstract: (265 Views) |
Introduction: Menopause and aging affect energy homeostasis by changing body composition and energy balance. Tamoxifen (TAM) is a non-steroidal anti-estrogenic compound that has estrogenic and non-estrogenic effects on several physiological systems of the body. The aim of the study is to determine the TAM’s central mechanism of effect on the brain responsiveness to leptin in young and aged mice.
Methods and Materials: Young (4 months old Intact and OVX) and aged (20 months old) female mice were challenged with a high-fat diet (HFD) for 12 weeks. Then they were divided into six groups young+oil, young+TAM, young-OVX+oil, young-OVX+TAM, aged+oil, and aged+TAM. After four weeks of treatment with TAM, the animals’ responsiveness to leptin was assessed by measuring leptin sensitivity indices as appetite control neuropeptides and p-STAT3.
Results: Findings showed that TAM, reduced body weight and energy intake in young mice. Also, leptin injection only increased α-MSH neuropeptide in TAM-treated young mice. Furthermore, it was found that TAM increased leptin sensitivity by increasing p-STAT3 levels in young groups. Conclusions: Overall, the results showed that TAM could not improve leptin sensitivity in aged mice, while TAM, increased leptin sensitivity in young mice. Therefore, it can be concluded that the function of TAM in the brain's responsiveness to leptin is age-dependent.
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| Keywords: Tamoxifen, aging, high-fat diet, Leptin responsiveness, Brain neuropeptides |
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Full-Text [PDF 314 kb]
(46 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2023/12/17 | Accepted: 2023/08/23 | Published: 2023/08/23
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