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Celecoxib suppresses neuroinflammation and oxidative stress-mediated apoptosis in rotenone-induced rat model of Parkinson’s disease
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Enam Alhagh Charkhat Gorgich   , Houman Parsaie , Seyed Jamal Hosseini , Maryam Sarbishegi |
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Abstract: (280 Views) |
Introduction: Parkinson’s disease (PD) is one of the most common neurodegenerative movement-related disturbances characterized by the degeneration of dopaminergic neurons with uncertain underlying mechanisms, which can be modeled by the rotenone (ROT). Neuroinflammation and oxidative stress (OS) are the most possible hypotheses to create this condition. Thus, the aim of this study was to evaluate the effects of celecoxib (CLX) on the ROT-induced rat model of PD.
Methods and Materials: In this experimental study, thirty-two male Sprague-Dawley rats were randomly classified into 4 groups (n = 8 rats/group) in the following order: control, sham, PD (2.5 mg/kg/48 hours ROT subcutaneously), CLX+PD (20 mg/kg/24 daily orally CLX+2.5 mg/kg/48 hours ROT). After 28 days of the experiment, the rats were sacrificed and their brain was removed. Then histological (Nissl staining) and biochemical assessments (total antioxidant capacity (TAC) were carried out and malondialdehyde (MDA) levels were measured. The data were analyzed by ANOVA test.
Results: The biochemical assessments showed TAC was significantly increased in the CLX+PD group compared with the PD group, whereas MDA was decreased in the CLX+PD group compared with the PD group (p<0.01). We found a significant decrease in the number of dopaminergic cells in substantia nigra pars compacta (SNc) in the PD group (p<0.001), and treatment with CLX markedly increased dopaminergic neurons in CLX+PD compared to the PD group (p<0.01).
Conclusion: Our findings indicated that CLX treatment can effectively improve the antioxidant defense system. These findings suggest that CLX plays a neuroprotective role in the inhibition of oxidative stress and neuroinflammation.
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| Keywords: Parkinson’s disease, Celecoxib, Oxidative stress, Rotenone, Dopaminergic neurons |
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Full-Text [PDF 393 kb]
(46 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2023/12/15 | Accepted: 2023/08/23 | Published: 2023/08/23
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