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The effect of low- and high-frequency electrical stimulation of the olfactory bulb on seizure severity and synaptic currents in kindled male rats
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Parisa Zareie   , Amir Shojaei , Yaghoub Fathollahi , Mohammad Reza Raoufy , Javad Mirnajafi-Zadeh |
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Abstract: (275 Views) |
Introduction: Deep brain stimulation (DBS) is an alternative treatment for drug-resistant temporal lobe epilepsies. Although high-frequency DBS got FDA approval for the treatment of epilepsy, the DBS target and the parameters of DBS for exerting the best therapeutic effect have remained poorly understood. In the present study, we investigated the anticonvulsant effects of low- and high-frequency stimulation (LFS and HFS) of the olfactory bulb on kindled seizures.
Methods and Materials: Rats underwent a kindling procedure using semi-rapid (6 stimulations per day) electrical stimulation of the hippocampal CA1 region. Full-kindled animals received either LFS (1 Hz) or HFS (130 Hz) at 5 min, 6 h, 24 h, and 30 h after the last kindling stimulation. Then, a kindling stimulation was applied, and the seizure behavior and afterdischarges were evaluated. In addition, excitatory and inhibitory postsynaptic currents (EPSC and IPSC) were recorded in the pyramidal neurons of the hippocampal CA1 region by whole-cell patch clamp technique.
Results: Overall, applying DBS in the OB at both low and high frequencies alleviated the seizure severity in the hippocampal-kindled rats. Furthermore, applying both LFS and HFS to the olfactory bulb of full-kindled animals led to a decrease in the amplitude, area, and rise slope of EPSCs and an increase in the amplitude, area, and rise slope of IPSCs. Interestingly, the excitation-to-inhibition ratio decreased following both LFS and HFS applications in kindled animals.
Conclusion: The olfactory bulb is a potential DBS target for the treatment of epilepsy. In addition, applying DBS at both low and high frequencies exerts similar anticonvulsant effects, showing that LFS may also be as effective as HFS in epilepsy treatment.
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| Keywords: Epilepsy, Deep brain stimulation, Olfactory bulb, Hippocampus, Seizure |
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Full-Text [PDF 262 kb]
(44 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2023/12/9 | Accepted: 2023/08/23 | Published: 2023/08/23
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