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Cross-talk between carbon monoxide and hydrogen sulfide on the CBS expression and oxidative stress induced by Chronic Kidney Disease in the brain of rat
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Zeinab Hamidizad   , Mehri Kadkhodaee , Farzaneh Kianian , Mina Ranjbaran , Behjat Seifi |
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Abstract: (305 Views) |
Introduction: neurotoxicity is a severe complication of chronic kidney disease (CKD). Accumulation of uremia leads to oxidative stress, inflammation and destruction of the blood-brain barrier. Carbon monoxide (CO) and hydrogen sulfide (H2S) have been shown to have anti-inflammatory, anti-apoptotic, and anti-proliferative properties. The aims of present study are evaluating the protective effects of CO-releasing molecule (CORM3) and H2S donor (NaHS) on oxidative stress induced by CKD in the hippocampus and prefrontal cortex by considering interaction CO and H2S on CBS expression.
Methods and Materials: sixty rats were divided into six experimental groups: sham, five-sixth (5/6) nephrectomy (CKD), CKD+H2S donor (NaHS), CKD+CO-releasing molecule (CORM3), CKD+NaHS and zinc protoporphyrin IX (Znpp), CKD+CORM3 and amino-oxy acetic acid (AOAA). At the end of the twelfth week after the induction of 5.6 nephrectomy, blood samples from the inferior vena cava were accumulated to measure kidney function indicators, BUN and plasma creatinine. In order to check the level of CBS enzyme expression, the right hippocampus and the medial prefrontal section were placed in a microtube. In order to measure MDA levels and SOD enzyme activity, left hippocampus and medial prefrontal were removed and kept in liquid nitrogen. Another part of left hippocampus and medial prefrontal tissue was fixed with 10% formalin after brain tissue perfusion for histological studies.
Results: CORM3 or NaHS significantly compensated deficits in the antioxidant defense mechanisms, suppressed lipid peroxidation that induced by CKD. In addition, CORM3 or NaHS significantly improved CBS expression which were reduced by CKD. However, improving effects of CORM3 on antioxidant defense mechanisms, lipid peroxidation and CBS expression were prevented by AOAA and as well as reciprocally improving effects of NaHS all indices were prevented by Znpp.
Conclusions: this study demonstrated that formation of CO and H2S were interdependently improved CKD-induced oxidative stress, which may be through increased expression of CBS.
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| Keywords: Oxidative stress, Carbon monoxide, Hydrogen sulfide, expression of CBS, Chronic kidney disease |
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Full-Text [PDF 261 kb]
(49 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2023/12/7 | Accepted: 2023/08/23 | Published: 2023/08/23
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