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Investigation of the Effect of Nano-Chrysin on Hormonal Changes, Oxidative Damage, and Pathological Changes in Thyroid Tissue of Male Rats Exposed to Chlorpyrifos
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Behzad Mesbahzadeh   , Tahereh Farkhondeh , Fatemeh Ahrari Moghadam |
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Abstract: (293 Views) |
Introduction: Organophosphate pesticides are recognized as environmental pollutants that can cause metabolic disturbances. Metabolic disorders involve increased oxidative stress parameters as one of the contributing factors. The aim of this study was to measure thyroid hormones, including T4, T3, TSH, and changes in oxidative stress parameters {malondialdehyde (MDA), glutathione (GSH), nitric oxide (NO), and superoxide dismutase (SOD) enzyme}, as well as histopathological changes in the thyroid gland of male Wistar rats exposed to chlorpyrifos and nano-chrysin.
Methods and Materials: In this study, 36 male Wistar rats were divided into 6 groups (n=6) and studied for 15 days. The groups included: control with 0.5 ml of chlorpyrifos solvent (olive oil), chlorpyrifos alone at a dose of 30 mg/kg, chlorpyrifos plus nano-chrysin at different doses (CPF+NCH5, CPF+NCH10), and nano chrysin alone at different doses (NCH5, NCH10). The materials were administered by Gavage. The thyroid gland of the animals was evaluated for antioxidant enzyme activity and histopathological changes, while serum samples were evaluated for thyroid hormones. Data were analyzed using mean, standard error, and statistical analysis.
Results: The results showed an increase in T3 and T4 concentrations in all groups compared to the control group, while TSH did not show a significant change. Treatment with nano-chrysin and chlorpyrifos alone had no effect on the level of oxidative biomarkers. Furthermore, histopathological changes were observed in all groups.
Conclusion: Oral consumption of nano chrysin cannot prevent the toxic effects of high doses of chlorpyrifos. |
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| Keywords: Chrysin, Oxidative stress, Thyroid, Wistar rats. |
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Full-Text [PDF 256 kb]
(42 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2023/12/4 | Accepted: 2023/08/23 | Published: 2023/08/23
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