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Isolation of Mucosal and Bulbar Olfactory Ensheathing Cells Based on a Novel Approach
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Fatemeh Tirgar   , Zahra Azizi , Mahmoudreza Hadjighassem |
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Abstract: (286 Views) |
Introduction: In addition to being frequently used in transplant studies, olfactory ensheathing cells (OECs) within the olfactory mucosa (OM) and olfactory bulb (OB), may be used as carriers to deliver therapeutic agents to specific target areas. These cells that encompass the unmyelinated axons of the olfactory neurons, have an accessible source for isolation, high migratory capacity and ability to secrete anti-inflammatory and neurotrophic factors. The mentioned features making them reasonable candidates for cell therapy. Although recent advancements in OEC isolation techniques heralded a new era in the field, the viability rate and efficacy of purification are still need to be considered. The most widely used OEC isolation strategies can be classified according to their adhesive properties, especially for isolating OECs from the OB. Considering the invasive nature of harvesting OECs from the human OB, highly efficient purification from the OM may be clinically beneficial.
Methods and Materials: In this study, we isolated and compared OECs from both OB and OM of rat, due to their different adherence characteristics. By immunocytochemistry and western blot analysis, specific markers of OEC cells were identified with NGFRp75 and S100β antibodies. OECs morphology and viability were tracked over time using microscopy and MTT assay.
Results: We discovered that using our proposed strategy, OECs can be purified from the OM as efficiently as the OB. OECs from both sources exhibited high levels of NGFRp75 and S100β expression, although the S100β expression was higher in OEC preparations derived from the OM (P<0.05). Furthermore, there was no significant difference in cell viability between the two sources.
Conclusion: Therefore, OECs extracted from the OM are obtained in a non-invasive manner and are clinically suitable for transplantation studies.
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| Keywords: Olfactory ensheathing cells, Cell purification, Olfactory bulb, Olfactory mucosa |
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Full-Text [PDF 324 kb]
(45 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2023/11/9 | Accepted: 2023/08/23 | Published: 2023/08/23
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