|
The effect of cyclosporine on asymmetric antibodies and serum transforming growth factor beta1 in abortion-prone model of mice CBA/J x DBA/2
|
Seyed Mohammad Moazzeni   , Bizhan Sadighi-Moghaddam |
|
|
|
Abstract: (5355 Views) |
| Introduction: One of the common causes of recurrent spontaneous abortion is interference of immunological factors. Administration of cyclosporine, as an immunosuppressive drug, has reportedly reduc the rate of abortion in abortion-prone model of mice CBA/J mated DBA/2 male mice. On the other hand, asymmetric antibodies and TGF-β1 have been considered as an important factor in successful pregnancy outcomes. Intravaginal administration of TGF-β has also been reported to reduce the resorption rate in the same mouse model. In this study, the immunomodulatory effects of cyclosporine on serum level of asymmetric antibodies (AAbs) and T cell growth factor beta (TGF-β1) were investigated.
Material and Methods: Female CBA/J mice were mated with male DBA/2. The pregnant females were divided into two groups: 1- test group received intraperitoneal injection of 1mg/kg cyclosporine (n=7), 2- control group received intraperitoneal injection of Phosphate Buffered Saline (PBS) (n=7). Both groups received treatments on the 5th day of pregnancy. Serum samples were collected on the 13.5th day of pregnancy, when the percentage of abortion was also calculated.
Results: The percentage of AAbs and abortion rate were significantly reduced in cyclosporine group (P0.05).
Conclusion: Our study showed that despite the significant reduction in percentage of AAbs and no significant difference in serum level of TGF-β1, the abortion rate was markedly reduced in cyclosporine group. So other mechanisms of immunomodulation and reduction of abortion rate except asymmetric antibodies and TGF-β1 should be involved in this mouse model following cyclosporine administration. |
|
| Keywords: Recurrent Spontaneous Abortion, Asymmetric Antibodies, Cyclosporine |
|
|
Full-Text [PDF 792 kb]
(1211 Downloads)
|
Type of Study: Research |
Subject:
پژوهشي
Received: 2015/01/13 | Accepted: 2015/02/9 | Published: 2015/06/14
|
|
|
|
|
|
|
| Send email to the article author |
|
|
|
| Add your comments about this article |
|
|
|
