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Effect of hydro-alcoholic extract of Vaccinium arctostaphylos on insulin release from rat-isolated langerhans islets
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Reza شفیعینیک   , Seyyed Mohammad Reza پریزاده , Neda ذکائی , Ahmad قربانی |
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Abstract: (12383 Views) |
| Introduction: Vaccinium arctostaphylos is one of the medicinal plants used in the treatment of diabetes. But the mechanisms responsible for its antidiabetic action have not been well defined. This study was undertaken to investigate the possible effect of V. arctostaphylos on insulin release from rat-isolated Langerhans islets. Materials and Methods : The islets were incubated in the presence of 3 or 10 mM glucose with or without milrinone (0.1 and 0.01%) and hydro-alcoholic extract of V. arctostaphylos (0.1 and 0.1%) for 60 min. The concentration of insulin was measured in samples of incubation medium. Results: The increase of glucose concentration in incubation medium from 3 to 10 mM resulted in a significant increase of insulin secretion. Milrinone at 0.1 mM significantly increased insulin release evoked by 10 mM glucose. However, none of the extract concentrations affected the insulin release. Conclusion: Our data demonstrated that V. arctostaphylos had no insulinotropic property and its effects on diabetes mediated by other mechanisms which should be clarified in future studies. |
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| Keywords: Vaccinium arctostaphylos, Hydro-alcoholic extract, Insulin, Langerhans islets, Rat |
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Full-Text [PDF 148 kb]
(3131 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2011/06/14 | Accepted: 2015/09/3 | Published: 2015/09/3
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