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Construction of a ceRNA network in subgroups of human cardiomyocytes affected by myocardial infarction using single-cell gene expression data
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Saeideh Jafarinejad-Farsangi   , Morteza Hadizadeh |
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Abstract: (225 Views) |
Introduction: Single-cell based transcriptome studies in human heart tissue following myocardial infarction have shown the existence of three distinct subgroups of cardiomyocytes, namely vCM1 (non-stressed cardiomyocytes), vCM2 (pre-stressed cardiomyocytes), and VCM3 (stressed cardiomyocytes), in the regions of the myogenic, transition, and ischemic zones, each displaying a different gene expression profile.
Methods and Materials: Given the importance of non-coding RNAs, such as myomiRNAs (heart- and muscle-specific miRNAs) and lncRNAs (long non-coding RNAs), in gene regulation, the objective of this study was to establish a ceRNA network (lncRNA-myomir-mRNA) in the aforementioned subgroups of cardiomyocytes. The targets of myomiRs and lncRNAs were downloaded from miRTarbase and Targetscan databases, and the shared differentially expressed miRNAs and their targeted myomiRs were identified using Venny tool 2.1. The final ceRNA network was constructed using Cytoscape 3.30.
Results: A small network consisting of seven myomiR target genes and one lncRNA was found in non-stressed cardiomyocytes. No ceRNA network was identified in pre-stressed cardiomyocytes, but a complex network of eight myomiRs, their target genes, and nine lncRNA was found in stressed cardiomyocytes located in the ischemic regions of the heart tissue, highlighting the importance of non-coding RNAs in gene regulation under ischemic conditions.
Conclusion: Investigation of downstream pathways revealed that myomiRs and lncRNAs regulate cellular junctions, angiogenesis, and immune and inflammatory responses. |
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| Keywords: single-cell gene expression, ceRNA network, myomiRs, lncRNAs, cardiomyocyte |
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Full-Text [PDF 262 kb]
(28 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/02/9 | Accepted: 2023/08/23 | Published: 2023/08/23
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