|
Anti-inflammatory effects of leaf and seed extracts of Plantago major on acetic acid-induced ulcerative colitis in rats: relation to cytokines
|
Armita Farid   , Mohammad Sheibani , Asie Shojaii , Mitra Noori , Manijeh Motevalian |
|
|
|
Abstract: (221 Views) |
Introduction: The Plantago major (P.major) has been traditionally used in Iranian herbal medicine to treat diarrhea and wound-healing. In this study, we aimed to evaluate the anti-inflammatory effects of leaf and seed extracts of P. major in rats with acetic acid-induced ulcerative colitis (UC).
Methods and Materials: 49 rats were randomly divided into seven groups. UC was induced in all groups except the control (vehicle) group through a single intra-rectal administration of 2ml of 4% acetic acid. The damage group did not receive any treatment after UC induction. The remaining five groups received daily injections (i.p.) of seed and leaf extracts of P.major (400 mg/kg and 700 mg/kg), and sulfasalazine (400 mg/kg), respectively for seven consecutive days. The rats’ rectum was surgically removed and evaluated for macroscopic and microscopic damage. Tissue levels of IL-1β, IL-6, IL-10, and TNF-α, PGE2, MPO, and MDA were measured using ELISA method.
Results: The high dose of leaf extract caused a significant reduction in ulcer index and histopathologic damage as well as the tissue levels of IL-1β, IL-6, TNF-α, PGE2, MPO, and MDA compared to the damage group. The low dose of leaf extract also caused a significant reduction in macroscopic and histopathologic damages, ulcer index, and IL-1β and PGE2 levels compared to the damage group. The seed extract in two used doses caused a modest reduction in the histopathological damages and ulcer index compared to the damage group, but the levels of the measured inflammatory markers were not significantly changed by the seed extract.
Conclusion: P.major leaf extract is effective in the reduction of inflammation and mucosal damage in rats with UC, especially when administered in high doses. P.major seed extract has minimal protective effects in UC. This justifies the traditional use of P. major leaves for inflammation-based diseases. |
|
| Keywords: Ulcerative colitis, P.major, Inflammation, Rats |
|
|
Full-Text [PDF 337 kb]
(29 Downloads)
|
Type of Study: Research |
Subject:
General
Received: 2024/02/9 | Accepted: 2023/08/23 | Published: 2023/08/23
|
|
|
|
|
|
|
| Send email to the article author |
|
|
|
| Add your comments about this article |
|
|
|
