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Effect of glutamine and metformin on the reproductive system dysfunction of diabetic male mice induced by methylglyoxal
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Maryam Kheirollahi Khorasani   , Akram Ahangarpour , Shahnaz Mojadami |
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Abstract: (275 Views) |
Introduction: Reproductive dysfunctions is one of diabetic important complications. This study investigated the effect of glutamine and metformin on male reproductive system in methylglyoxal (MGO) induced diabetic mice.
Methods and Materials: 40 male NMRI mice, one-month-old, weighing 20-25g were divided into 4 groups (n=10): sham, MGO (600 mg/Kg/day), MGO+metformin 150 mg/kg/day, MGO+glutamine (500 mg/kg/day). Methylglyoxal, metformin and glutamine administered orally in 30 days. At the 31th day of the study, blood samples, testes and epididymis separated.
Results: Blood glucose and insulin levels in MGO group were higher than sham group (p<0.001). These values decreased with metformin (p<0.001) and glutamine (p<0.01) treatment. Glutamine reduced body weight (p<0.01) and therefor testicular weight (p<0.05) compared to the sham group. Testis width and volume decreased in the MGO receiving mice (p<0.05). Superoxide dismutase decreased in diabetic mice (p<0.05) and both treatments improved it (p<0.05). Malondialdehyde enhanced in MGO group (p<0.001), and reduced with both treatments (p<0.001). Testosterone (p<0.001), and sperm count (p<0.01) decreased in the diabetic mice, both treatments recovered testosterone level (p<0.001), while metformin could not improve sperm count. Luteinizing hormone increased in diabetic mice (p<0.001) and had improved in treatment groups (p<0.01). Follicle stimulating hormone did not show differences in all groups.
Conclusion: Reproductive system affects by MGO induced diabetes. MGO increases oxidative stress, decrease antioxidant capacity and reduces sex hormones. Glutamine and metformin treatment improved them. Finally, in some parameters metformin, and in others glutamine performed better.
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| Keywords: Diabetes, Methylglyoxal, Glutamine, Metformin, Reproductive system, Oxidative stress |
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Full-Text [PDF 286 kb]
(42 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/01/16 | Accepted: 2023/08/23 | Published: 2023/08/23
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