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Effect of soluble Carcinoembryonic antigen on myogenic and enterocytic differentiation
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Abbas پاکدل   , Fakhraddin نقیب الحسینی |
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Abstract: (11575 Views) |
| Introduction: Carcinoembryonic antigen (CEA), a glycophosphatidyl inositol (GPI)
anchored glycoprotein, is over-expressed in various cancers, including
colorectal carcinomas. Inhibition of cell differentiation can cause cancer.
Previous studies show transfection and over-expression of CEA gene in myoblasts
or colonocytes leads to inhibition of cell differentiation. We investigated
whether soluble CEA has a role in inhibition of cell differentiation. Materials and Methods: Monolayer
cultures of L6, C2C12, Caco-2 and CHO cell lines were grown in dulbecco's modification
of eagle's medium (DMEM) containing 10% fetal bovine serum (FBS). For
differentiation induction C2C12 and L6 cell lines media, was changed to DMEM
containing 2% horse serum. CEA concentration was measured in harvested media by
ELISA assay. H&E staining, CK assay and RT-PCR for myogenin gene were used
for C2C12 differentiation experiments. Results: The
results showed although commercial pure CEA and LS-180 conditioned media
(contain high level of CEA) have inhibitory effects on Caco-2 differentiation,
but it can also inhibit differentiation of control groups. We observed the
effect of high concentration of CEA (5μg/ml) on L6 differentiation is identical
with the effect of BSA as control. C2C12 differentiation was inhibited in
response to LS-180 conditioned media at morphological level and myogenin expression. CK activity was significantly lower in LS-180
conditioned media (P= 0.0012) and CHO (P= 0.0002) conditioned media groups in
comparison to control group. Conclusion: Our finding shows soluble CEA antigen did not
have a significant effect on cell differentiation. |
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| Keywords: Carcinoembryonic antigen, Colorectal neoplasms, Myogenin |
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Full-Text [PDF 1163 kb]
(2196 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2012/07/14 | Accepted: 2013/04/8 | Published: 2013/04/8
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