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The Effect of Nigella sativa Against Kidney Damage in Rats with Rhabdomyolysis
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Ahmad Reza Aminian   , Sara Hosseinian , Abolfazl Khajavi Rad , Samira Shahraki , Fatemeh Salami , Mohammad Taghi Khoshniat |
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Abstract: (268 Views) |
Introduction: Rhabdomyolysis, or destruction of skeletal muscle, is the release of the contents of skeletal muscle cells into the plasma, which can be filtered through the glomeruli and lead to acute renal failure by various mechanisms.One of the experimental models for inducing rhabdomyolysis in laboratory animals is intramuscular injection of glycerol.Oxidative stress plays an important role in the pathophysiology of kidney damage caused by rhabdomyolysis.
Methods and Materials: Forty male Wistar rats were randomly divided into five groups: control, rhabdomyolysis, Nigella sativaextract (200 & 400 mg/kg) + rhabdomyolysis, thymoquinone (15 mg/kg) + rhabdomyolysis. The duration of the study was seven days and on the third day of the study, 50% glycerol (10 ml/kg) was injected intramuscularly into both legs. Serum and urine samples were collected at the beginning and end of the study and serum samples were collected on day four of the study (24 hours after glycerol injection). On the last day of the study, the kidneys were collected and animals were humanly killed.
Results: In the rhabdomyolysis group, on day four (24 hours after glycerol injection), serum levels of CPK, urea and creatinine showed a significant increase compared to the control group. On the last day of the study, in rhabdomyolysis group serum CPK levels did not differ significantly, but serum urea and creatinine concentrations, GFR and urea clearance showed a significant decrease and urinary output a significant rise compared to the control animals. MDA concentration of kidney tissue in the rhabdomyolysis group showed a significant increase compared to the control group.
Conclusion: The present study showed that total Nigella sativaextract and thymoquinone had a good protective effect on renal function in animals with rhabdomyolysis. It is possible that part of these beneficial effects of Nigella sativaextract and thymoquinone is due to their antioxidant effect.
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| Keywords: rhabdomyolysis, nigella, acute kidney failure |
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Full-Text [PDF 254 kb]
(34 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/02/5 | Accepted: 2023/08/23 | Published: 2023/08/23
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