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Effects of saffron aqueousextract and low frequency electromagnetic field on angiogenesison in a Wistar rat aortic ring model
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| Safa Moshtagh, Javad Baharara *, Saede Zafar-balanejad, Tayebe Ramezani |
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Abstract: (5932 Views) |
| Introduction: Angiogenesis is required for embryogenesis and many physiological and
pathological conditions such as tumor growth. The effects of electromagnetic
fields on cell development and growth have been investigated. In this
experimental study, the synergic effects of saffron aqueous extract and
low-frequency electromagnetic field on angiogenesis were investigated on rat
aortic ring. Materials and Methods: In this experimental study, Wistar rat aorta was divided into pieces 1 mm
and cultured in collagen matrices. On day 3, following observed sprouting
angiogenesis the samples were classified into 8 groups: Control group, the first
sham-exposed group (treated with PBS), the second sham-exposed group (treated
with electromagnetic field off), and the third sham-exposed group treated with PBS
and electromagnetic field off, the experimental groups 1 and 2 treated with 200
Gauss field for 2 hours and the concentrations of 200 and 300 µg/ml of saffron,
respectively and the experimental groups 3 and 4 treated with 200 Gauss field
for 3 hours and 200 and 300 µg/ml of saffron, respectively. All samples were
photographed by invert microscope immediately and also 24 hours after the
treatment. The number and length of vessels was determined by Image J software. Results: There was no significant differences between the control samples and the laboratory control samples 1, 2 and 3
with regards to average number and length of (P>0.05). However, the number
of vessels in experimental groups 2 and 4 showed a significant reduction (P |
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| Keywords: Angiogenesis inhibitors, Crocus, Aorta, Endothelial cells, Electromagnetic field, Rats |
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Full-Text [PDF 391 kb]
(1227 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2013/10/31 | Accepted: 2014/04/13 | Published: 2014/06/11
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