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Dopamine stimulates proliferation of Toxoplasma gondii tachyzoites in human fibroblast cells
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Setayesh Yasami Khiabani   , Maryam Mostafavi , Zohreh Askary pour , Jalal Babaie , Majid Golkar |
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Abstract: (222 Views) |
Introduction: Toxoplasma gondii is an obligate intracellular parasite that causes toxoplasmosis in humans. T. gondii leads to increased production of dopamine in the host's brain, which in turn causes various neurological disorders, including schizophrenia. Studies have suggested that an imbalance in the brain concentration of dopamine or its signaling plays a role in the occurrence of psychotic disorders. In this study, the effect of dopamine in the infection of HHF-1 cells by Tachyzoite- the first developmental stage of toxoplasmosis- was investigated.
Methods and Materials: The effect of different concentrations of dopamine on tachyzoite proliferation in HHF-1 was investigated. Then, the effect of selective antagonists of D1-like receptors (SCH-23390), D1-like receptors (sulpiride) and the combination of these two antagonists were used to inhibit the effect of dopamine in order to determine which receptors mediate the effect of dopamine. RT-qPCR method and microscopic counting were used to confirm parasite infection.
Results: The results showed that dopamine significantly (P<0.05) stimulates infection with the tachyzoite form of the parasite and increases the growth and proliferation of tachyzoites in HHF-1 cells. SCH-23390 significantly reduced the growth and proliferation of tachyzoites in HFF-1 cells (P<0.05), while sulpiride did not block the effect of dopamine. Also, the combination of two antagonists, SCH-23390 and sulpiride, significantly reduced tachyzoite proliferation in HFF-1 cells (P<0.05).
Conclusion: The findings of this research showed that dopamine has a facilitating role in T. gondii cell infection and exerts its effect through D1 type receptors. The results of this study help to understand how psychotic disorders occur in people with T. gondii. The results might help to design and develop effective drugs against toxoplasma and neurobehavioral disorders. |
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| Keywords: Toxoplasma gondii, dopamine, antagonist, HHF-1 |
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Full-Text [PDF 253 kb]
(28 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/02/27 | Accepted: 2023/08/10 | Published: 2023/08/10
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