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Effects of Alamandine on Monocrotaline-Induced Pulmonary Hypertension in Rats
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Ava Soltani Hekmat   , Freshteh Amini , Amirali Ebrahim Babaei , Hamideh Shahbazi , Ali Abbasi , Zahra Gholami , Kazem Javanmardi |
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Abstract: (199 Views) |
Introduction: Pulmonary arterial hypertension (PAH) is a severe and often fatal disease that is associated with oxidative stress and inflammation. Alamandine known for its antioxidative, anti-inflammatory, and antifibrotic effects, has been investigated in this study to determine if it has protective effects against PAH induced by monocrotaline (MCT), and if these effects are associated with oxidative stress, inflammatory factors, and inducible nitric oxide synthase (iNOS).
Methods and Materials: Rats were administered MCT (40 mg/kg) on day 0 and then received alamandine (50 mg/kg/day) via mini-osmotic pumps for 21 days starting one day later. Hemodynamic parameters, electrocardiograms, superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), inflammatory cytokines (TNF-α, IL-1β, NF-κB), iNOS, and MrgD receptor expression in lung tissue were evaluated at the end of the 21-day period. The MrgD receptor was quantified through immunofluorescent staining, and the histopathology of lung tissues was evaluated using hematoxylin and eosin staining.
Results: The results showed that alamandine treatment significantly improved hemodynamic parameters, oxidative stress markers, inflammatory factors, and echocardiographic data. Furthermore, treatment with alamandine decreased the levels of iNOS. Additionally, alamandine treatment decreased the expression levels of MrgD receptors in the lung tissue of MCT-induced PAH.
Conclusion: In summary, this study indicates that alamandine has protective effects against monocrotaline-induced PAH, and these effects may be attributed to the inhibition of oxidative stress, inflammatory parameters, and iNOS. |
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| Keywords: Alamandine, Monocrotaline, Pulmonary Hypertension, Oxidative Stress, Immunohistochemistry |
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Full-Text [PDF 324 kb]
(25 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/02/26 | Accepted: 2023/08/10 | Published: 2023/08/10
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