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Dexamethasone release for hyaluronic acid microparticle and gelatin hydrogel for sciatic tissue regeneration
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Kazem Javanmardi   , Hamideh Shahbazi , Amirali Ebrahim Babaei , Ava Soltani Hekmat , Zahra Gholami , Ali Abbasi , Arash Goodarzi , Mehdi Khanmohamadi |
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Abstract: (251 Views) |
Introduction: Biodegradable microparticle is a useful vehicle in the controlled release of bioactive molecules for the practical use of the device in drug delivery, tissue engineering, and biopharmaceutical applications.
Methods and Materials: We developed dexamethasone (Dex) loaded in tyramine substituted hyaluronic acid microparticle (Dex HA-Tyr Mp) by horseradish peroxidase (HRP) mediated crosslinking using the microfluidic device as well as gelatin (Gela) with crosslinked with proanthocyanidin (PA) as a constructive hydrogel bed in the repair of sciatic tissue.
Results: It was found that simultaneous usage of crosslinked Gela-based hydrogel and Dex HA-Tyr Mp improved the physical properties of the hydrogel, including mechanical resistance and stability. Also, the designed composite provides a sustained release system for Dex delivery to the surrounding environment which proved the applicability of fabricated hydrogel composite for sciatic nerve tissue engineering and regeneration. The encapsulated cells were viable and showed suitable growth ability and morphogenesis during the extended time of incubation in Gela-Ca-PA/HA-Tyr Mp hydrogel compared to control conditions. Histological analysis revealed a significant increase in the number of axons in injured sciatic nerves following treatment by Dex-loaded hyaluronic acid microparticle and Gela-Ca-PA hydrogel buck compared to negative control groups.
Conclusion: In summary, the results showed that composite biodegradable microparticles containing Dex and bulk Gela-Ca-PA are an effective approach for improving nerve regeneration in rats.
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| Keywords: Hyaluronic acid microparticle, Proanthocianate, hydrogelation, Dexamatasone release, Sciatic tissue engineering |
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Full-Text [PDF 255 kb]
(42 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/02/20 | Accepted: 2023/08/10 | Published: 2023/08/10
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