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Evaluation of Dracocephalum kotschyi on isolated heart contractions
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Baharh Arzi   , Hassan Sadraei , Afsaneh Yegdaneh |
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Abstract: (225 Views) |
Introduction: Dracocephalum kotschyi Boiss (Labiatae), traditionally has been used for the treatment of rheumatism and respiratory disorders. Previous pharmacological studies have demonstrated antispasmodic effect of D. kotschyi on smooth muscle including, ileum, uterus, bladder and trachea. However, so far, the pharmacological effect of D. kotschyi extract on cardiovascular system has not been investigated. Therefore this research evaluated pharmacological action of D. kotschyi extract and essential oil on rat isolated heart contraction.
Methods and Materials: Hydroalcoholic extract and essential oil were prepared by maceration and hydrodistillation techniques respectively. Wistar rat was killed and heart was dissected out and immersed in ice cold oxygenated modified Krebs solution. The whole heart was setup in an organ bath filled with the Krebs solution under 1.5g tension and continuously gassed with carbogen. Myocardium contractions were recorded on Harvard Universal Oscillographs. Effect of the extract or the essential oil were determined by adding directly into the organ bath using two folds increment in concentration. The control tissues were treated with equivolume of the extract vehicle. Nifedipine was used as standard drugs. Myocardium contraction were measured as amplitude of the contraction and results expressed as percentage of recorded contractions prior to addition of drug. The data expressed as mean±SEM (n=6). Results were compared using ANOVA or Student’s t-test.
Results: Whole isolated heart suspended in the organ bath exhibited regular beating which was potentiated with addition of adrenaline (50µM). Cumulative addition of the essential oil (20-400µg/ml) reduced amplitude of recorded contractions in a concentration-dependent manner while the hydroalcoholic extract had no inhibitory effect on the spontaneous contraction of rat isolated heart. As expected, nifedipine reduced myocardium contractions.
Conclusion: Unlike the hydroalcoholic extract of D. kotschyi, the essential oil exhibited negative inotropic effect on the isolated rat heart. |
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| Keywords: Dracocephalum kotschyi, heart, Hydroalcoholic extract, essential oil, adrenaline |
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Full-Text [PDF 313 kb]
(32 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/02/11 | Accepted: 2023/08/23 | Published: 2023/08/23
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