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The Study of Cholinesterase Enzyme Serum Level Changes and Brain Expression Level of Cathepsin D in LPS-Induced Depression in Male Mice
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Rahineh Nomani Lafmejani   , Mahtab Zare , Zohreh Fazollahi , Mehrdad Roghani |
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Abstract: (217 Views) |
Introduction: Depression is a common disorder, especially in developed countries. Functional changes of cholinesterase and cathepsin D are involved in pathogenesis of some brain disorders. Until now, exact association of these factors with depression has not been determined. This study was conducted to evaluate the changes of serum cholinesterase and brain cathepsin D in lipopolysaccharide (LPS)-induced model of depression in male rats.
Methods and Materials: Male rats (n=48) were divided into 2 groups, each in two subgroups of control and LPS. For induction of depression, LPS (0.5 mg/kg; i.p.) was injected 24 h before the experiments. Open field, forced swimming, and tail suspensions tests was used for behavioral assessment. Finally, serum cholinesterase and brain cathepsin D expression was determined using Western blot method.
Results: LIP injection significantly decreased travelled distance in open field test (P<0.05) and increased immobility duration in forced swimming and tail suspension tests (P<0.01). In addition, serum cholinesterase showed a significant decrease in LPS subgroups versus control (P<0.05) and cathepsin D expression significantly decreased in LPS-treated rats as compared to control (P<0.05).
Conclusion: Our data showed that LPS could induce a valid model of depression and changes of cholinesterase and cathepsin D are in part involved in development of its complication.
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| Keywords: Depression, Lipopolysaccharide, Cholinesterase, Cathepsin D, Open Field Test |
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Full-Text [PDF 268 kb]
(33 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/02/10 | Accepted: 2023/08/23 | Published: 2023/08/23
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