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Finding of Ginkgo biloba as a GABAA agonist and its role on the rat sleep, in silico and in vivo studies
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Farideh Bahrami   , Mir Behrad Aghazadeh Ghadim , Mohsen Sisakht , Mahdi Niazi , Alireza Shahriari |
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Abstract: (220 Views) |
Introduction: Sleep disorders are a widespread health issue affecting millions of people globally and can have a significant impact on an individual's quality of life. Herbal medications with less side effects than chemical medicines have been preferred. The aim of this study, is to evaluate the plant compounds entered in PubChem database according to their non-toxicity and drug ability with the best interaction to the GABAA receptor by in silico method and then to test the selected compound with in vivo method.
Methods and Materials: In this study, all 2845 known phytochemical compounds in the Pabchem database were examined by in silico method. Using the GABAA receptor to molecular docking, as the best compound with the lowest ΔG for all three GABAA receptor binding sites, Ginkgetin, was chosen. For the in vivo study, 14 Wistar rats received Gincobiloba extract and vehicle for 21 days. The sleep of the animals was recorded by electrophysiology and examined with MATLAB software.
Results: The results of this study determined that the duration of NREM, and REM sleep were significantly increased in the group treated with Ginkgo biloba extraction compared to vehicle. The REM sleep has increased from 4.8% to 18.7%, while the NREM sleep increased significantly from 37/89 to 47/19% (P<0.001).
Conclusion: The results of this study suggest that Ginkgo biloba have potential as a treatment for sleep disorders. According to the in silico study this increase of sleep may be related to the better affinity of Ginkgetin the common component of Gincobiloba plant to the GABAA receptors. |
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| Keywords: Ginkgo biloba, GABAA receptors, Sleep, In silico |
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Full-Text [PDF 336 kb]
(27 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/02/9 | Accepted: 2023/08/23 | Published: 2023/08/23
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