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Prangos ferulaceae (L.) ameliorates the behavioral, histopathological and molecular brain disorders in valproic acid-induced rat model autism
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Maryam Saadat   , Abbas Ali Taherian , Mohammad Reza Aldaghi , Payman Raise-Abdullahi , Abbas Ali Vafaei , Hamid Reza Sameni |
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Abstract: (226 Views) |
Introduction: Prenatal exposure to valproic acid (VPA) may enhance the risk of autism spectrum disorder (ASD) in children. This study investigated the effect of Prangos ferulaceae (L.) on VPA-induced ASD in rats.
Methods and Materials: Pregnant rats received VPA (600 mg/kg, i.p.) or saline on gestational day 12.5 (E 12.5). Starting from the 30th postnatal day (PND 30), the pups were intraperitoneally (i.p.) administered Prangos ferulaceae (PF, 100 and 200 mg/kg), or the vehicle, daily until PND 58 On PND 30 and 58, various behavioral tasks were used to evaluate pups, including the open field, elevated plus-maze, hot-plate, and rotarod test. On PND 65, the animals were euthanized, and their brains were removed for histopathological and biochemical assay.
Results: Prenatal exposure to VPA caused significant behavioral changes in the offspring, reversed by administering an extract of Prangos ferulaceae (L.). Additionally, prenatal VPA administration resulted in increased levels of malondialdehyde (MDA) and deficits in antioxidant enzyme activities in the hippocampus, including catalase (CAT) and glutathione (GSH), ameliorated by PF. Likewise, postnatal treatment with PF improved VPA-induced dysregulation of Bax and Blc2 in the hippocampus and reduced neuronal death in CA1, CA3, and dentate gyrus.
Conclusion: The findings of this study suggest that postnatal administration of PF can prevent VPA-induced autism spectrum disorder (ASD)-like behaviors by exhibiting anti-apoptotic and anti-oxidant properties. Therefore, PF may have the potential as an adjunct in the management of ASD.
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| Keywords: Autism, Valproic acid, Oxidative stress, Hippocampus, Prangos ferulaceae (L.) |
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Full-Text [PDF 271 kb]
(37 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/02/5 | Accepted: 2023/08/23 | Published: 2023/08/23
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