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Acute Anti-Inflammatory Effects of Neostigmine in the Rat Air Pouch Model of Inflammation
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Tahereh Eteraf-Oskouei   , Iman Haj-Hasan , Parvin Ghadimi , Moslem Najafi |
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Abstract: (231 Views) |
Introduction: A bidirectional interaction between the nervous system and immune system was becoming increasingly well documented. The aim of the present study was to evaluate the effect of neostigmine on the inflammatory parameters in a rat model for rheumatoid arthritis, namely air pouch model of inflammation.
Methods and Materials: To induce air pouches, wistar rats (200-250 g) were anesthetized, and then 20 ml and 10 ml of sterile air were injected subcutaneously on the back of the animals on days 0 and 3, respectively. On day 6, inflammation was induced by injection of 2 ml of carrageenan 1% into pouches. Normal saline as control and neostigmine (70, 140, and 200 μg/kg) was administered intraperitoneally concurrently with carrageenan. After 6 hours, the rats were sacrificed and the pouches were opened with a surgical scissors; pouch fluid was collected in order to determine exudates volume and cells were counted using cell counter. Pouches were dissected out and the weight determined.
Results: Neostigmine (70 μg/kg) reduced leukocyte accumulation (P<0.05), exudate volume (P<0.001), and granulation tissue weight (P<0.001) compared to the carrageenan control group. Inflammatory parameters were increased by neostigmine 200 μg/kg (P<0.05).
Conclusion: From this study, it may be concluded that in the rat air pouch inflammatory model, neostigmine at different doses had different and contradictory effects on peripheral inflammation.
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| Keywords: Neostigmine, Inflammation, Air pouch, Carrageenan, Rat |
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Full-Text [PDF 325 kb]
(36 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/02/2 | Accepted: 2023/08/23 | Published: 2023/08/23
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