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The preconditioning effect of kombucha tea on cerebral ischemia/reperfusion injury in global cerebral ischemia
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Fatemeh Eskandari Vaezi   , Fezzeh Hosseinzadeh |
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Abstract: (227 Views) |
Introduction: Cerebral ischemia is the most common cause of neurological impairment and brain edema formation. Recent studies have shown that neutrophils are the first peripheral immune cells migrating through inflated blood vessels, soft meninges, or the choroid plexus and accumulating in the ischemic regions of the brain following brain stroke and involved in the ischemic cascade. Neutrophils lead to secondary damage by releasing proinflammatory factors, proteases, matrix metalloproteinases, and reactive oxygen species (ROS). Kombucha tea (KT) is a fermented beverage with potential antioxidant compounds and various health effects. This study evaluated the impact of KT pretreatment on brain edema, neutrophils, and oxidative stress in the global brain ischemia model.
Methods and Materials: Adult male Wistar rats were divided into; sham, ischemic, and KT-treated-ischemic group. KT was administered two weeks before inducing global cerebral ischemia at dosage (1 ml/kg/day, gavage). Brain ischemia was induced by blocking the common carotid arteries for one hour, followed by 24 hours of reperfusion. Brain edema was determined by the dry-wet method. Malondialdehyde (MDA) and total antioxidant capacity (TAC) were measured in serum and brain by the ELISA method, and the neutrophils were counted by a Cell Counter.
Results: Our results showed that KT pretreatment significantly reduced brain edema and the MDA levels in the blood and brain. The TAC levels increased in the blood and brain. KT pretreatment reduced the neutrophils in the peripheral blood.
Conclusion: Our data demonstrated that KT pretreatment reduced blood-brain edema. It seems that the beneficial effects of it may be through decreasing lipid peroxidation and Neutrophils production and increasing antioxidant defense capacity in ischemic conditions.
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| Keywords: Antioxidant, Cerebral ischemia, Kombuchatea, Brain edema |
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Full-Text [PDF 267 kb]
(31 Downloads)
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Type of Study: Applicable |
Subject:
General
Received: 2024/02/2 | Accepted: 2023/08/23 | Published: 2023/08/23
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