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The protective effects of gallic acid and SGK1 inhibitor on oxidative stress and cardiac damage in an isolated heart model of ischemia/reperfusion injury in rat
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Faramarz Souri   , Mohammad Badavi , Mahin Dianat , Ali Mard , Alireza Sarkaki |
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Abstract: (222 Views) |
Introduction: Oxidative stress and the serum and glucocorticoid-induced Kinase 1 gene (SGK1) perform a central role in the consequences of ischemia in the heart. This research aimed to investigate the effect of coadministration of gallic acid and the GSK650394 (as SGK1 gene inhibitor) on the ischemic complications of a rat model of cardiac ischemia/reperfusion (I/R) injury.
Methods and Materials: Sixty male Wistar rats were divided into 6 groups with or without pretreatment with gallic acid for 10 days. After that, the heart was isolated and perfused with Krebs-Henseleit solution. A 30 min of ischemia was performed followed by a 60 min reperfusion. In 2 groups, GSK650394 was infused 5 minutes before ischemia induction. Ten min after reperfusion commencement, cardiac marker enzymes (CK-MB, LDH, and cTn-I) activities were measured in the cardiac perfusate. At the end of reperfusion, the activity of antioxidant enzymes (Catalase, Superoxide dismutase, and Glutathione peroxidase), lipid peroxidation (MDA), total antioxidant capacity (TAC), intracellular reactive oxygen species (ROS), infarct size, and SGK1 gene expression were measured in the heart tissue.
Results: The results indicated that dual therapy with both drugs significantly improved endogenous antioxidant enzymes activity and TAC more than each drug alone. However, the heart marker enzymes (CK-MB, LDH, and cTn-I), MDA, ROS, infarct size, and SGK1 gene expression were reduced significantly compared to the ischemic group.
Conclusion: The results of this study suggest that concomitant administration of both drugs in the case of cardiac I/R injury may have a more beneficial effect than each one alone.
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| Keywords: Gallic Acid, Serum-glucocorticoid regulated kinase 1 (SGK1), GSK650394, Oxidative Stress, Reperfusion Injury |
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Full-Text [PDF 271 kb]
(38 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/01/19 | Accepted: 2023/08/23 | Published: 2023/08/23
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