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Effect of zinc oxide nanoparticles on hepatic ischemia-reperfusion injury: role of miR-125b expression in possible underlying mechanisms
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Feryal Savari   , Seyed Ali Mard , Anahita Rezaie , Mojtaba Kalantar |
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Abstract: (280 Views) |
Introduction: The present study examined the protective effects of ZnO-NPs on hepatic ischemia-reperfusion injury (HIRI) in rats and their possible underlying mechanism.
Methods and Materials: Thirty-eight male Wistar albino rats were randomly divided into six groups (n=8) including the sham group that received intraperitoneally normal saline solution (Sham), the ehpatic ischemia-reperfusion injury group (HIRI), the control group pre-treated with 5 mg/kg zinc oxide nanoparticles (ZnO-NPs) for 3 consecutive days without surgery (ZnO5), the control group pre-treated with 10 mg/kg ZnO-NPs for 3 consecutive days without surgery (ZnO10), HIRI group pre-treated with 5 mg/kg ZnO-NPs for 3 consecutive days before surgery (HIRI+ZnO5), and HIRI group pre-treated with 10 mg/kg ZnO-NPs for 3 consecutive days before surgery (HIRI+ZnO10). One hour after reperfusion, biochemical, and histopathological evaluations and expression of miR-125b were performed.
Results: Administration of ZnO-NPs caused significant improvement in the elevated serum concentration of aminotransferase enzymes (ALT and AST), total oxidant status (TOS), Malondialdehyde (MDA), and improvement in the liver histopathology and increasing TNF-α, IL-6, and NF-κB levels in liver tissue compared to HIRI group (p < 0.05). In addition, administration of ZnO-NPs could increase expression in miR-125 in liver tissue compared to the HIRI group (p < 0.05).
Conclusions: The administration of ZnO-NPs could improve the liver damage induced by ischemia-reperfusion via enhancing miR-125b expression and suppressing oxidative stress and inflammatory cytokines.
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| Keywords: Hepatic ischemia-reperfusion injury, ZnO-NPs, miR-125b, Oxidative stress, Inflammatory response |
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Full-Text [PDF 329 kb]
(36 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/01/5 | Accepted: 2023/08/23 | Published: 2023/08/23
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