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Proliferation and differentiation of adipose tissue derived mesenchymal stem cell on natural human kidney scaffold
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Mehdi Kheirandish   , Samira Shahraki , Abolfazl Khajavi Rad , Alireza Ebrahimzadeh Bideskan , Mohammad Asl Zare |
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Abstract: (241 Views) |
Introduction: Various diseases can lead to end stage renal disease (ESRD) that is a progressive loss of kidney function with a high rate of morbidity and mortality. Renal transplantation is currently the most effective treatment for ESRD. However, the number of available donor kidneys is drastically insufficient to meet the demand. For this reason, tissue engineering by way of seeding cells on scaffold offers a great potential to increase the pool of donated organs for kidney transplantation.
Methods and Materials: In the current work, adipose tissue derived MSCs were seeded onto the triton-treated cortex scaffolds for 3 weeks. Proliferation and differentiation of MSCs into kidney-specific cell types on cortex scaffolds were then analyzed by H&E and IHC staining.
Results: After reccelularization of the renal human cortex scaffolds with MSCs, cell attachment and migration into the triton-treated scaffold were confirmed histological studies results as compared with non-seeded scaffolds at same days. Furthermore, the results of IHC staining showed that Aquaporin 2 (AQP2) expressed in seeded human cortex scaffold compared with non-seeded cortex scaffolds after 7, 14, and 21 days from cell seeding.
Conclusions: In this regard, these natural cortex scaffolds supported the growth of MSCs and could also induce their differentiation into epithelial and endothelial cells. |
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| Keywords: Kidney, Recellularization, Mesenchymal stem cells, Scaffold, Aquaporin 2 |
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Full-Text [PDF 366 kb]
(47 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2024/01/4 | Accepted: 2023/08/23 | Published: 2023/08/23
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