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Cannabinoid Type 1 Receptor Antagonist Impaired Spatial Memory and Increased the Tau Gene Expression in Animal Model of the Alzheimer's disease
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Mahdi Goudarzvand   , Bahareh Tavakoli-Far , Parviz Fallah , Parisa Darabi , Roham Mazloom , Elaheh Gholami Roudmajani |
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Abstract: (268 Views) |
Introduction: Alzheimer's disease is a neurodegenerative disease characterized by the accumulation of two different proteins and microscopic manifestations: the β-amyloid and the tau protein. The present study aimed to scrutinize the effects of bilateral administration of the cannabinoid receptor antagonist (AM251) in the hippocampus on spatial memory and tau gene expression in an Alzheimer's disease model.
Methods and Materials: β-amyloid toxin was injected bilaterally into the hippocampus of Wistar male rats to induce Alzheimer's disease, and the rats were then categorized into the control group (receiving distilled water as β-amyloid toxin solvent), lesion group (receiving β-amyloid), β-amyloid + DMSO group (as antagonist solvent), and AM251 antagonist receiving groups. The Morris water maze test was used to confirm the development of the model. During the training course of the Morris test, the antagonist of cannabinoid 1 receptor AM251 was injected bilaterally into the hippocampus for four days at doses of 5, 25, and 100 ng. To assess the animals' spatial memory, the parameters of the distance traveled by the animals, the time spent to reach the hidden platform, the speed of the animals moving through the Morris test, and the direction evaluation of the mRNA expression in real-time were analyzed.
Results: The spatial memory indices in the Morris water test increased after the injection of β-amyloid toxin; the AM251 cannabinoid antagonist relatively elevated the level of memory impairment. The evaluation of the mRNA expression of tau protein revealed an increase in its level following an injection of β-amyloid toxin. However, there was no significant difference between the cannabinoid antagonist and β-amyloid groups.
Conclusion: The results indicate the destructive effect of beta-amyloid toxin on spatial memory, as well as the positive role of the cannabinoid system in memory formation and consolidation. However, further studies are warranted in this regard.
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| Keywords: Alzheimer's disease, AM251, tau protein, spatial memory |
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Full-Text [PDF 392 kb]
(42 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2023/12/13 | Accepted: 2023/08/23 | Published: 2023/08/23
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