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Investigating the comparative effect of 5-FU on the expression level of BAX and Bcl2 genes in high glucose and normal glucose mediums in human colorectal cancer cells (HT-29) under hypoxic conditions
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Parisa Kadkhoda   , Elham Chamani , Mohsen Khorashadizadeh |
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Abstract: (269 Views) |
Introduction: Colorectal cancer (CRC) is the third most common cancer worldwide. Chemotherapy is a widely used treatment for CRC. 5-FU has been the most significant in treating CRC among the various chemotherapeutic agents available. However, many studies have investigated the antiproliferative effect of 5-FU in colorectal cancer, the comparative effect of 5-FU on the expression level of BAX and Bcl2 genes in high and normal glucose medium in human colorectal cancer cells (HT-29) under hypoxic conditions has not been studied.
Methods and Materials: HT-29 cells were cultured in DMEM medium supplemented with 10% FBS and 1% antibiotic. Then, cells were treated with different concentrations of 5-FU (3.125, 6.25, 12.5, 25, 50μM) and Cocl2 (50,100,150,200μM) in both high and normal glucose mediums for 72 hours. The MTT assay was used to investigate the cytotoxicity of the 5-FU and Cocl2 in both mediums. Furthermore, BAX and Bcl2 expressions were assessed by qRT-PCR.
Results: The MTT assay results demonstrated that the IC50 value for cells treated with 150 μM concentration of Cocl2 in high glucose medium was higher than normal glucose medium (IC50 for high glucose and normal glucose; 28.4856 μM,20.5740 μM). This study also revealed that IC50 value in hypoxic conditions, in both high and normal glucose medium, was higher than in normoxic conditions. Additionally, the BAX/Bcl2 ratio in the normal glucose group was higher than in the high glucose group.
Conclusion: Glucose concentration may play a critical role in the cytotoxicity of 5-FU in CRC. These findings highlight the need for further research to explore the relationship between glucose concentration and the cytotoxicity of 5-FU. This could have significant consequences for the treatment of colorectal cancer.
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| Keywords: Colorectal Cancer, 5-FU, High and normal glucose, Cocl2, BAX, Bcl2 |
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Full-Text [PDF 327 kb]
(37 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2023/12/8 | Accepted: 2023/08/23 | Published: 2023/08/23
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