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The effects of quercetin on the expression of circular RNAs circPAN3, circRalgapa1, and circSLC8a1 in the hearts of rats with myocardial infarction
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Mohammad Mojtaba Farazi   , Farzaneh Rostamzadeh , Saeideh Jafarinejad-Farsangi , Sedigheh Gharbi |
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Abstract: (241 Views) |
Introduction: Quercetin, a member of the flavonoid family, has protective and improving effects on cardiovascular diseases (CVDs), including myocardial infarction (MI). However, the underlying molecular mechanisms are still being investigated. Meanwhile, a new group of non-coding RNAs known as circular RNAs has been found to play a role in the pathogenesis and progression of CVDs and MI. In this study, we examined the expression patterns of three circular RNAs (circRalgapa1, circSLC8a1, and circPAN3) and their relationship with the healing effects of quercetin in rats with MI. Male rats were divided into four groups: a control (sham) group, a one-day MI group, a two-week MI group, and an MI group treated with quercetin (30 mg/kg, intraperitoneal injection, every other day for two weeks). MI was induced by closing the left anterior descending coronary artery (LAD), and heart tissue was collected two weeks later under full anesthesia after checking heart function indicators. Real-time PCR was used to measure gene expression levels.
Result: The expression of circRalgapa1 and circSLC8a1 in the heart tissue of the two-week and one-day MI groups decreased significantly (P<0.05), while the expression of circPAN3 increased significantly (P<0.05). However, only the expression of circPAN3 showed a significant change (P<0.05) after quercetin treatment.
Conclusion: These results suggest that quercetin may reduce the expression of circular RNA circPAN3, consistent with its function in the heart. Nonetheless, further investigation is needed to fully elucidate the downstream mechanisms of circPAN3.
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| Keywords: myocardial infarction, circular RNA, quercetin, rat |
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Full-Text [PDF 253 kb]
(35 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2023/12/7 | Accepted: 2023/08/23 | Published: 2023/08/23
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