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Investigating the effects of curcumin and Niosome curcumin on alterations in NF-κB gene expression and oxidative stress parameters in Alzheimer's rats
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Elahe Asani   , Homeira Hatami , Somayeh Hatami |
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Abstract: (272 Views) |
Introduction: Alzheimer's is a neurodegenerative disease, and exposure to aluminum chloride causes the activation of the TLR4/NF-κB signaling pathway and increases the expression of TLR4 and NF-κB inflammatory genes, neuroinflammation, oxidative stress, and finally Alzheimer's. Curcumin antioxidant improves Alzheimer's disease by reducing oxidative stress and neuroinflammation, but due to curcumin's low biological activity, nanoparticles such as niosome have been used. In this study, the antioxidant effect of curcumin and niosome curcumin on alterations in NF-κB gene expression and oxidative stress parameters in the prefrontal cortex of Alzheimer's rats was investigated.
Materials and methods: In this study, 56 male Wistar rats were randomly divided into 7 groups (n=8): 1) control (28 days of saline), 2) sham (14 days of ethanol), 3) Alzheimer (aluminum chloride 2.4 mg/kg; 28 days), 4) Alzheimer + Curcumin, 5) Curcumin (20 mg/kg; 14 days), 6) Niosome Curcumin (20 mg/kg; 7 days), 7) Alzheimer + Niosome Curcumin (injection All drugs were intraperitoneal). After the end of the treatment period, the animals were sacrificed and their prefrontal cortex was removed to measure Nuclear Factor kappa B (NF-κB) gene expression using RT-PCR and to analyze oxidative stress parameters including Malondialdehyde (MDA) and Superoxide Dismutase (SOD). One-way ANOVA was used to analyze the data.
Results: The levels of MDA (unlike SOD) and the level of NF-κB gene expression in Alzheimer's groups receiving curcumin and niosome curcumin are significant reduction compared to the Alzheimer's group (p<0.05, p<0.0001). Also, the levels of SOD in Alzheimer's groups receiving curcumin and Niosome Curcumin increased significantly compared to Alzheimer's group (p<0.0001).
Conclusion: showed that NF-κB gene expression level, and MDA levels increased during induction of Alzheimer's disease with aluminum chloride and treatment with curcumin and niosome curcumin improves these effects in Alzheimer's rats.
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| Keywords: Alzheimer, Malondialdehyde, Superoxide Dismutase, Nuclear factor kappa B, Niosome |
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Full-Text [PDF 388 kb]
(48 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2023/11/16 | Accepted: 2023/08/23 | Published: 2023/08/23
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